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LEADER 03419ctm 2200493 i 4500

001 ocm25749519;

003 OCoLC;

005 20250422153006.0;

008 920501s1973 xx a bm 000 0 eng d;

035 a| (Sirsi) o25749519;

035 a| (OCoLC)25749519;

049 a| EREE;

050 4 a| QP88.5b| .S38x 1973;

100 1 a| Sawyer, Linwood McAuley,d| 1949- ,e| author.?| UNAUTHORIZED;

245 12 a| A fine structural analysis of eight-day chicken comb and comb- feather chimeras /c| by Linwood McAuley Sawyer.;

264 0 c| 1973.;

300 a| 54 leaves :b| illustrations ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.A.c| East Carolina Universityd| 1973;

500 a| Submitted to the faculty of the Department of Biology.;

500 a| Advisor: Irvin E. Lawrence, Jr.;

520 2 a| Epithelial-mesenchymal interactions in 8-day chick skin were studied in chimeras prepared for electron microscopy. Chimeric grafts were formed by enzymatic separation and recombination of comb epidermis on feather dermis and feather epidermis on comb dermis. The chimeras were grown on the chorioallantoic membrane tor 24, 48, and 72 hours. Enzymatic separation of the tissues removed the basal lamina. The dermal fibroblasts did not retain their characteristic epidermally oriented pattern. Microfilament bundles in the fibroblasts were affected, and collagen was disorganized. After recombination the basal lamina was gradually reformed, dermal cells resumed their normal oriented pattern and anchor filaments were also modified. Since acid mucopolysaccharides have been implicated in epithelial-mesenchymal interactions, ruthenium red was used to demonstrate their presence. Acid mucopolysaccharides were observed in the basal lamina of 8-day comb epidermis, but were not apparent in the basal lamina of 8-day feather epidermis. These morphological findings provide further evidence for an inductive role for epidermis at o days. This inductive activity may be expressed through differences in the matrix material.;

504 a| Includes bibliographical references (leaves 25-28).;

650 0 a| Tissuesx| Analysis.=| ^A75258;

650 0 a| Poultryx| Histology.=| ^A70230;

650 7 a| Tissuesx| Analysis.2| fast0| (OCoLC)fst01151500;

655 7 a| dissertations.2| aat0| (CStmoGRI)aatgf300028029;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Lawrence, Irvin E.,c| Jr.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/11478;

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949 a| Click on web addressw| asish| hsl111;

994 a| C0b| ERE;

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998 a| 416950;

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A fine structural analysis of eight-day chicken comb and comb- feather chimeras / by Linwood McAuley Sawyer.

Author/creator	Sawyer, Linwood McAuley, 1949- author.
Other author	Lawrence, Irvin E., Jr., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	1973.
Description	54 leaves : illustrations ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Tissues--Analysis. Poultry--Histology.

Scope and content	Epithelial-mesenchymal interactions in 8-day chick skin were studied in chimeras prepared for electron microscopy. Chimeric grafts were formed by enzymatic separation and recombination of comb epidermis on feather dermis and feather epidermis on comb dermis. The chimeras were grown on the chorioallantoic membrane tor 24, 48, and 72 hours. Enzymatic separation of the tissues removed the basal lamina. The dermal fibroblasts did not retain their characteristic epidermally oriented pattern. Microfilament bundles in the fibroblasts were affected, and collagen was disorganized. After recombination the basal lamina was gradually reformed, dermal cells resumed their normal oriented pattern and anchor filaments were also modified. Since acid mucopolysaccharides have been implicated in epithelial-mesenchymal interactions, ruthenium red was used to demonstrate their presence. Acid mucopolysaccharides were observed in the basal lamina of 8-day comb epidermis, but were not apparent in the basal lamina of 8-day feather epidermis. These morphological findings provide further evidence for an inductive role for epidermis at o days. This inductive activity may be expressed through differences in the matrix material.
General note	Submitted to the faculty of the Department of Biology.
General note	Advisor: Irvin E. Lawrence, Jr.
Dissertation note	M.A. East Carolina University 1973
Bibliography note	Includes bibliographical references (leaves 25-28).
Genre/form	dissertations.
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

A fine structural analysis of eight-day chicken comb and comb- feather chimeras / by Linwood McAuley Sawyer.

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