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LEADER 03967ctm 2200577 i 4500

001 ocm26236438;

003 OCoLC;

005 20250422153006.0;

008 920722s1992 xx af bm 000 0 eng d;

035 a| (Sirsi) o26236438;

035 a| (OCoLC)26236438;

049 a| EREE;

050 4 a| QR69.A57b| P37x 1992;

100 1 a| Parker, Anita C.,e| author.?| UNAUTHORIZED;

245 14 a| The cloning and characterization of a cefoxitin resistance determinant in Bacteroides vulgatus CLA341 /c| by Anita C. Parker.;

264 0 c| 1992.;

300 a| vii, 69 leaves, 7 unnumbered plates :b| illustrations ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 1992;

500 a| Submitted to the faculty of the Department of Biology.;

500 a| Advisor: C. Jeffrey Smith;

520 3 a| A clinical isolate of Bacteroides vulgatus, strain CLA 341, was resistant to tetracycline (Tc) and S-lactam antibiotics including cefoxitin (Cf) a drug usually effective in the treatment of Bacteroides infections. The objectives of this study were to: (1) identify and characterize the mechanism of Cf resistance; (2) determine if the Cf resistant determinant was transmissible from B. vulgatus to other Bacteroides; (3) clone the cefoxitin resistance determinant. Cf resistance was shown to be mediated by a [Beta]-lactamase that transferred to other Bacteroides by a conjugation-like event when donor cells were pretreated with Tc. Progeny were resistant to Cf, Tc, or both drugs, however, only those resistant to both drugs could become conjugative donors in secondary matings. The structural gene of the [Beta]-lactamse was cloned from genomic libraries in Escherichia coli. The library clone containing the Cf resistance gene (cfxA) was detected after transfer to B. fragilis. Southern analysis of DNA from Cf and Cf/Tc resistant progeny revealed cfxA was encoded on chromosomal and not plasmid DNA. Generally, the gene inserted into the chromosome in one preferred site, however, single and multiple insertions at alternate sites were also observed. These results suggest that cfxA is located on a transposable element.;

504 a| Includes bibliographical references (leaves 62-69).;

650 0 a| Microorganismsx| Effect of antibiotics on.=| ^A96981;

650 0 a| Antibioticsx| Testing.=| ^A273942;

650 0 a| Beta lactamases.=| ^A119148;

650 0 a| Cloning.=| ^A8574;

650 0 a| Drug resistance in microorganisms.=| ^A718;

650 7 a| Antibioticsx| Testing.2| fast0| (OCoLC)fst00810437;

650 7 a| Beta lactamases.2| fast0| (OCoLC)fst00830898;

650 7 a| Cloning.2| fast0| (OCoLC)fst00864579;

650 7 a| Drug resistance in microorganisms.2| fast0| (OCoLC)fst00898704;

650 7 a| Microorganismsx| Effect of antibiotics on.2| fast0| (OCoLC)fst01019945;

655 7 a| dissertations.2| aat0| (CStmoGRI)aatgf300028029;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Smith, C. Jeffrey,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/12660;

949 a| Click on web addressw| asish| joyner101;

949 a| Click on web addressw| asish| hsl111;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 426222;

999 a| ASK AT SPECIAL COLLECTIONS DESKw| ASISc| 1i| 682042d| 9/27/2001l| JSCJTHDm| JOYNERr| Ys| Yt| JSCJTHDu| 9/27/2001x| ETDz| JSPECCOLLo| .STAFF. 0;

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The cloning and characterization of a cefoxitin resistance determinant in Bacteroides vulgatus CLA341 / by Anita C. Parker.

Author/creator	Parker, Anita C. author.
Other author	Smith, C. Jeffrey, degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	1992.
Description	vii, 69 leaves, 7 unnumbered plates : illustrations ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Microorganisms--Effect of antibiotics on. Antibiotics--Testing. Beta lactamases. Cloning. Drug resistance in microorganisms.

Summary	A clinical isolate of Bacteroides vulgatus, strain CLA 341, was resistant to tetracycline (Tc) and S-lactam antibiotics including cefoxitin (Cf) a drug usually effective in the treatment of Bacteroides infections. The objectives of this study were to: (1) identify and characterize the mechanism of Cf resistance; (2) determine if the Cf resistant determinant was transmissible from B. vulgatus to other Bacteroides; (3) clone the cefoxitin resistance determinant. Cf resistance was shown to be mediated by a [Beta]-lactamase that transferred to other Bacteroides by a conjugation-like event when donor cells were pretreated with Tc. Progeny were resistant to Cf, Tc, or both drugs, however, only those resistant to both drugs could become conjugative donors in secondary matings. The structural gene of the [Beta]-lactamse was cloned from genomic libraries in Escherichia coli. The library clone containing the Cf resistance gene (cfxA) was detected after transfer to B. fragilis. Southern analysis of DNA from Cf and Cf/Tc resistant progeny revealed cfxA was encoded on chromosomal and not plasmid DNA. Generally, the gene inserted into the chromosome in one preferred site, however, single and multiple insertions at alternate sites were also observed. These results suggest that cfxA is located on a transposable element.
General note	Submitted to the faculty of the Department of Biology.
General note	Advisor: C. Jeffrey Smith
Dissertation note	M.S. East Carolina University 1992
Bibliography note	Includes bibliographical references (leaves 62-69).
Genre/form	dissertations.
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

The cloning and characterization of a cefoxitin resistance determinant in Bacteroides vulgatus CLA341 / by Anita C. Parker.

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