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007 cr bn|||||||||;

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035 a| (Sirsi) o936178199;

035 a| (OCoLC)936178199;

049 a| EREE;

090 a| QR201.B8;

100 1 a| Atkinson, Xavier J.,e| author.?| UNAUTHORIZED;

245 10 a| Determining the chemical structure of brucebactin :b| the sole complex siderophore utilized by the pathogenic bacterium Brucella abortus /c| by Xavier J. Atkinson.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2016.;

300 a| 59 pages :b| illustrations (some color);

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 1.6Mb2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 a| Presented to the Faculty of the Department of Biology.;

502 b| M.S.c| East Carolina Universityd| 2016.;

500 a| Advisor: Eric Anderson.;

500 a| Title from PDF t.p. (viewed February 29, 2016).;

520 3 a| Brucellosis is a zoonotic bacterial disease that affects many around the world. There are over half a million new cases each year and prevalence rates in some areas of the world exceed 10 new cases per 100,000 in the population. It is one of the world's most common zoonotic diseases. In cattle, brucellosis can result in spontaneous abortion in females and sterility in males. In humans, brucellosis causes the disease Undulant fever. Undulant fever is characterized by a cyclic fever, and can include other symptoms such as headaches, malaise, sweats, and muscle pains. The causative agent of brucellosis is Brucella abortus which is a Gram negative coccobacillus bacterium. Because this pathogen persists in an iron-limited environment within the host, it must produce iron capturing molecules called siderophores, to meet its metabolic needs. The structure of the major siderophore produced by the brucellae, called brucebactin, is currently not known, and the goal for this project was to determine its structure. Using thin layer chromatography (TLC), Mass Spectrometry, and NMR, new information about the compound was determined. TLC separation followed by Electron Impact Mass Spec analysis identified an iron-binding compound consisting of two molecules of 2,3-DHBA bound by a spermidine polyamine linker. A compound with the hypothesized structure of brucebactin was prepared by organic chemical synthesis, and found to have the same HPLC properties and mass spectral profile as the material isolated from bacterial culture.;

504 a| Includes bibliographical references.;

650 0 a| Brucellosis.=| ^A3503;

650 0 a| Brucella abortus.=| ^A784450;

650 0 a| Bacteriax| Morphology.=| ^A12914;

653 a| Biology;

653 a| Microbiology;

653 a| Chemistry;

653 a| brucebactin;

653 a| siderophore;

700 1 a| Anderson, Erice| degree supervisor.=| ^A696706;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/5112;

949 o| wjh;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 4356009;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 4356009-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 1/28/2016x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 4356009-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 1/28/2016x| ETDz| HERESOURCE;

Determining the chemical structure of brucebactin : the sole complex siderophore utilized by the pathogenic bacterium Brucella abortus / by Xavier J. Atkinson.

Author/creator	Atkinson, Xavier J. author.
Other author	Anderson, Eric degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2016.
Description	59 pages : illustrations (some color)
Supplemental Content	Access via ScholarShip
Subjects	Brucellosis. Brucella abortus. Bacteria--Morphology.

Summary	Brucellosis is a zoonotic bacterial disease that affects many around the world. There are over half a million new cases each year and prevalence rates in some areas of the world exceed 10 new cases per 100,000 in the population. It is one of the world's most common zoonotic diseases. In cattle, brucellosis can result in spontaneous abortion in females and sterility in males. In humans, brucellosis causes the disease Undulant fever. Undulant fever is characterized by a cyclic fever, and can include other symptoms such as headaches, malaise, sweats, and muscle pains. The causative agent of brucellosis is Brucella abortus which is a Gram negative coccobacillus bacterium. Because this pathogen persists in an iron-limited environment within the host, it must produce iron capturing molecules called siderophores, to meet its metabolic needs. The structure of the major siderophore produced by the brucellae, called brucebactin, is currently not known, and the goal for this project was to determine its structure. Using thin layer chromatography (TLC), Mass Spectrometry, and NMR, new information about the compound was determined. TLC separation followed by Electron Impact Mass Spec analysis identified an iron-binding compound consisting of two molecules of 2,3-DHBA bound by a spermidine polyamine linker. A compound with the hypothesized structure of brucebactin was prepared by organic chemical synthesis, and found to have the same HPLC properties and mass spectral profile as the material isolated from bacterial culture.
General note	Advisor: Eric Anderson.
General note	Title from PDF t.p. (viewed February 29, 2016).
Dissertation note	Presented to the Faculty of the Department of Biology.
Dissertation note	M.S. East Carolina University 2016.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

Determining the chemical structure of brucebactin : the sole complex siderophore utilized by the pathogenic bacterium Brucella abortus / by Xavier J. Atkinson.

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