The effects of prolactin on epidermal gylcoconjugates in the red-spotted newt, Notophthalmus viridescens viridescens, using lectin histochemistry / by Demetrice Ward.
| Author/creator | Ward, Demetrice L. author. |
| Other author | Singhas, Charles A., degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 1992. |
| Description | 80 leaves : illustrations ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | The present study uses lectin histochemistry to examine the extent to which prolactin (PRL)-induced integumental changes in red-spotted newts are due to alterations in the expression and distribution of glycoconjugates within the epidermis and epidermal glands. A panel of eight horseradish peroxidase labelled lectins (LTA, UEA-I, DBA, SBA, GSAI-B4, WGA, PNA, CON A), which bind specifically to terminal carbohydrate moieties of glycoproteins and glycolipids was chosen. The epidermis of PRL-injected newts showed a marked decrease in lectin binding for LTA, DBA, SBA, and PNA as compared to control salineinjected newts. The binding of WGA to sialic acid increased with PRL injection, while GSAI-B4 and CON A showed little to no change in binding. Temporal studies revealed that differential lectin binding occurred by day 6 in PRL-injected newts a well as the epidermis of freshly collected newts undergoing conditioning. Seasonal examination revealed similarities in the expression and distribution of epidermal glycoconjugates between freshly collected winter animals and laboratory conditioned animals that had received PRL. Together, these findings demonstrate that prolactin may bring about integumental changes in the red-spotted newt by affecting the expression and distribution of both secretory and structural glycoconjugates. |
| General note | Submitted to the faculty of the Department of Biology. |
| General note | Advisor: Charles A. Singhas |
| Dissertation note | M.S. East Carolina University 1992 |
| Bibliography note | Includes bibliographical references (leaves 68-72). |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
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|---|---|---|---|---|
| Joyner | University Archives | ASK AT SPECIAL COLLECTIONS DESK | ✔ Available | Request Material |
| Electronic Resources | Access Content Online | ✔ Available |