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001 on1021186501;

003 OCoLC;

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006 m o d ;

007 cr unu||||||||;

008 180201s2017 ncua obm 000 0 eng d;

035 a| (Sirsi) o1021186501;

035 a| (OCoLC)1021186501;

049 a| EREE;

090 a| QP624.5.R48;

100 1 a| Knuckles, Christopher I.,e| author.?| UNAUTHORIZED;

245 10 a| Investigating the genetic interaction between DNA replication proteins Mcm10 and RecQ4 in Drosophila melanogaster /c| by Christopher I. Knuckles.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2017.;

300 a| 68 pages :b| illustrations (chiefly color);

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 8.856 MB2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2017;

500 a| Presented to the faculty of the Department of Biology;

500 a| Advisor: Tim W. Christensen;

500 a| Title from PDF t.p. (viewed April 24, 2018).;

520 3 a| Necessary to the survival of cellular life is proper replication and maintenance of the genome. Replication proteins Mcm10 and RecQ4 have well-characterized essential roles in assembly, initiation, proper functioning of the eukaryotic replication machinery, and genome stability. Mcm10 enables efficient assembly of the pre-replication complex while RecQ4 delivers essential proteins forming that complex; both of which are pivotal for origin firing. Recent studies suggest that Mcm10 is not required for RecQ4 chromatin localization or association with the CMG complex, conflicting with a previous report that Mcm10 mediates the interaction between RecQ4 and Mcm2-7 in an S-CDK dependent manner. We have found that a homozygous mutation of RecQ4 is lethal in fruit flies unless they possess a C-terminal domain (CTD) truncation of Mcm10, suggesting that Mcm10 and RecQ4 exhibit a genetic interaction. Here, we investigate how an Mcm10/RecQ4 genetic interaction might be important for organism viability and to facilitate critical replication states - like those found in larval brain development and adult oogenesis. We also investigate the individual and combined roles of Mcm10 and RecQ4 in genome stability. The Mcm10 CTD truncation rescue of lethal RecQ4 phenotypes can reveal much about how the cooperative roles of these proteins affect DNA replication and responses to DNA damage, but this requires further investigation. Our research aims to explore the nature of the Mcm10/RecQ4 genetic interaction by conducting genetic investigations, proliferation assays, and double strand break (DSB) studies in the Drosophila larval brain and adult ovary. We propose that Mcm10 and RecQ4 genetically interact to facilitate DNA replication and genome stability in Drosophila melanogaster.;

504 a| Includes bibliographical references.;

650 0 a| DNA replication.=| ^A825742;

650 0 a| Chromosomal proteins.=| ^A1233778;

650 0 a| Drosophila melanogaster.=| ^A86209;

650 0 a| DNA damage.=| ^A273441;

653 a| Mcm10;

653 a| RecQ4;

700 1 a| Christensen, Tim,e| degree supervisor.=| ^A1389912;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/6533;

949 o| wjh;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 4814991;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 4814991-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 2/1/2018x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 4814991-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 2/1/2018x| ETDz| HERESOURCE;

Investigating the genetic interaction between DNA replication proteins Mcm10 and RecQ4 in Drosophila melanogaster / by Christopher I. Knuckles.

Author/creator	Knuckles, Christopher I. author.
Other author	Christensen, Tim, degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2017.
Description	68 pages : illustrations (chiefly color)
Supplemental Content	Access via ScholarShip
Subjects	DNA replication. Chromosomal proteins. Drosophila melanogaster. DNA damage.

Summary	Necessary to the survival of cellular life is proper replication and maintenance of the genome. Replication proteins Mcm10 and RecQ4 have well-characterized essential roles in assembly, initiation, proper functioning of the eukaryotic replication machinery, and genome stability. Mcm10 enables efficient assembly of the pre-replication complex while RecQ4 delivers essential proteins forming that complex; both of which are pivotal for origin firing. Recent studies suggest that Mcm10 is not required for RecQ4 chromatin localization or association with the CMG complex, conflicting with a previous report that Mcm10 mediates the interaction between RecQ4 and Mcm2-7 in an S-CDK dependent manner. We have found that a homozygous mutation of RecQ4 is lethal in fruit flies unless they possess a C-terminal domain (CTD) truncation of Mcm10, suggesting that Mcm10 and RecQ4 exhibit a genetic interaction. Here, we investigate how an Mcm10/RecQ4 genetic interaction might be important for organism viability and to facilitate critical replication states - like those found in larval brain development and adult oogenesis. We also investigate the individual and combined roles of Mcm10 and RecQ4 in genome stability. The Mcm10 CTD truncation rescue of lethal RecQ4 phenotypes can reveal much about how the cooperative roles of these proteins affect DNA replication and responses to DNA damage, but this requires further investigation. Our research aims to explore the nature of the Mcm10/RecQ4 genetic interaction by conducting genetic investigations, proliferation assays, and double strand break (DSB) studies in the Drosophila larval brain and adult ovary. We propose that Mcm10 and RecQ4 genetically interact to facilitate DNA replication and genome stability in Drosophila melanogaster.
General note	Presented to the faculty of the Department of Biology
General note	Advisor: Tim W. Christensen
General note	Title from PDF t.p. (viewed April 24, 2018).
Dissertation note	M.S. East Carolina University 2017
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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Investigating the genetic interaction between DNA replication proteins Mcm10 and RecQ4 in Drosophila melanogaster / by Christopher I. Knuckles.

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