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003 OCoLC;

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035 a| (Sirsi) o29349675;

035 a| (OCoLC)29349675;

049 a| EREE;

050 4 a| RC771b| .H62 1993;

100 1 a| Hodges, Michael G.,e| author.?| UNAUTHORIZED;

245 14 a| The effect of colchicine (CC) on the plaque-forming cell response and serum antibody titer in pneumococcal polysaccharide (SSS-III) immunized BALB/c mice /c| by Michael G. Hodges.;

264 0 c| 1993.;

300 a| 60 leaves :b| illustrations ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 1993;

500 a| Submitted to the faculty of the Department of Biology.;

500 a| Advisor: A. Mason Smith;

520 3 a| The purpose of this thesis is to present data obtained from a study of the effects of the drug colchicine on the immune response to type three pneumococcal polysaccharide in BALB/c mice. A localized hemolysis-in-gel assay was used to determine the number of plaque-forming cells in the spleen of mice receiving the drug at various times relative to immunization. An enzyme-linked-immunosorbent assay was developed in order to determine humoral antibody titers in the immunized mice. Experimental protocols were designed to test the effect of colchicine on the major regulatory T-cells involved in the immune response to the polysaccharide antigen. The lectin concanavalin A was used in the study to activate T-suppressor and T-amplifier cells. A low dose priming technique was also used to produce over-expression of T suppressor cells specific for the antigen. The original hypothesis was that colchicine would act as an adjuvant, thereby increasing the number of antigen reactive B cells in the spleen as well as increasing the amount of antibody being produced. It was concluded that colchicine had an adjuvant effect on the cells in the regulatory circuit that were activated in a temporal fashion. Colchicine given at the same time as the antigen caused increased suppression since the T-suppressor cells were the first regulatory cells in the circuit. These results suggest that the T-amplifier cells may not be greatly influenced by the drug. A suboptimal dose of the antigen plus colchicine given at the same time produced a plaque-forming cell response equal to the response to an optimal dose of antigen. Serum antibody titers assayed five days post immunization remained relatively constant throughout the various experimental treatments. These findings are discussed in terms of possible regulatory mechanisms involved in the response to the bacterial antigen.;

504 a| Includes bibliographical references (leaves 56-60).;

650 0 a| Pneumonia, Pneumococcalx| Immunological aspects.=| ^A836123;

650 0 a| Colchicine.=| ^A321854;

650 0 a| Mice as laboratory animals.=| ^A848;

650 2 a| Colchicine.0| (DNLM)D003078=| ^A1462492;

655 2 a| Academic Dissertation.0| (DNLM)D019478?| UNAUTHORIZED;

650 7 a| Colchicine.2| fast0| (OCoLC)fst00866953;

650 7 a| Mice as laboratory animals.2| fast0| (OCoLC)fst01019388;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Smith, A. Mason,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/12502;

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The effect of colchicine (CC) on the plaque-forming cell response and serum antibody titer in pneumococcal polysaccharide (SSS-III) immunized BALB/c mice / by Michael G. Hodges.

Author/creator	Hodges, Michael G. author.
Other author	Smith, A. Mason, degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	1993.
Description	60 leaves : illustrations ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Pneumonia, Pneumococcal--Immunological aspects. Colchicine. Mice as laboratory animals. Colchicine.

Summary	The purpose of this thesis is to present data obtained from a study of the effects of the drug colchicine on the immune response to type three pneumococcal polysaccharide in BALB/c mice. A localized hemolysis-in-gel assay was used to determine the number of plaque-forming cells in the spleen of mice receiving the drug at various times relative to immunization. An enzyme-linked-immunosorbent assay was developed in order to determine humoral antibody titers in the immunized mice. Experimental protocols were designed to test the effect of colchicine on the major regulatory T-cells involved in the immune response to the polysaccharide antigen. The lectin concanavalin A was used in the study to activate T-suppressor and T-amplifier cells. A low dose priming technique was also used to produce over-expression of T suppressor cells specific for the antigen. The original hypothesis was that colchicine would act as an adjuvant, thereby increasing the number of antigen reactive B cells in the spleen as well as increasing the amount of antibody being produced. It was concluded that colchicine had an adjuvant effect on the cells in the regulatory circuit that were activated in a temporal fashion. Colchicine given at the same time as the antigen caused increased suppression since the T-suppressor cells were the first regulatory cells in the circuit. These results suggest that the T-amplifier cells may not be greatly influenced by the drug. A suboptimal dose of the antigen plus colchicine given at the same time produced a plaque-forming cell response equal to the response to an optimal dose of antigen. Serum antibody titers assayed five days post immunization remained relatively constant throughout the various experimental treatments. These findings are discussed in terms of possible regulatory mechanisms involved in the response to the bacterial antigen.
General note	Submitted to the faculty of the Department of Biology.
General note	Advisor: A. Mason Smith
Dissertation note	M.S. East Carolina University 1993
Bibliography note	Includes bibliographical references (leaves 56-60).
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

The effect of colchicine (CC) on the plaque-forming cell response and serum antibody titer in pneumococcal polysaccharide (SSS-III) immunized BALB/c mice / by Michael G. Hodges.

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