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LEADER 03223cam 2200529Ii 4500

001 on1105587088;

003 OCoLC;

005 20191023085849.0;

006 m o d ;

007 cr unu||||||||;

008 190624s2019 ncua obm 000 0 eng d;

035 a| (Sirsi) o1105587088;

035 a| (OCoLC)1105587088;

049 a| EREE;

090 a| QR82.B95;

100 1 a| Johnsrude, Matthew J.,e| author.?| UNAUTHORIZED;

245 14 a| The CDF-type metal efflux protein EmfA is essential for Brucella abortus 2308 resistance to manganese toxicity and is a critical virulence determinant in experimentally-infected mice /c| by Matthew J. Johnsrude.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2019.;

300 a| 99 pages :b| illustrations (chiefly color);

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 1.455 MB2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2019.;

500 a| Presented to the faculty of the Department of Biology;

500 a| Advisor: R. Martin Roop II;

500 a| Title from PDF t.p. (viewed October 15, 2019).;

520 3 a| Divalent cationic metals are important trace nutrients for all bacteria because metals serve as cofactors for proteins involved in numerous cellular functions. Despite the fact that metals play an essential role in bacterial physiology, metals become harmful to bacteria if accumulated in excess. To prevent metal-induced toxicity, bacteria employ a variety of factors, such as metal-responsive transcriptional regulators and metal efflux proteins, that function to maintain proper intracellular metal homeostasis. The Brucella strains are Gram-negative, pathogenic bacteria that cause the severe febrile zoonotic disease, brucellosis, and these bacteria require the metal manganese for growth and during host infection. While the manganese acquisition protein, MntH, is essential for the capacity of Brucella spp. to maintain sufficient manganese nutrition, the mechanisms by which these bacteria prevent manganese toxicity have not been determined. The studies of this thesis were performed to identify how Brucella spp. cope with manganese toxicity and whether these factors are essential for Brucella virulence.;

504 a| Includes bibliographical references.;

650 0 a| Brucella abortus.=| ^A784450;

650 0 a| Proteins.=| ^A2392;

650 0 a| Manganese.=| ^A89270;

650 0 a| Metalsx| Toxicology.=| ^A5194;

653 a| EmfA;

653 a| Mur;

700 1 a| Roop, R. Martin,c| II,e| degree supervisor.=| ^A1383281;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/7276;

949 o| wjh;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 5111069;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5111069-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 6/24/2019x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5111069-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 6/24/2019x| ETDz| HERESOURCE;

The CDF-type metal efflux protein EmfA is essential for Brucella abortus 2308 resistance to manganese toxicity and is a critical virulence determinant in experimentally-infected mice / by Matthew J. Johnsrude.

Author/creator	Johnsrude, Matthew J. author.
Other author	Roop, R. Martin, II, degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2019.
Description	99 pages : illustrations (chiefly color)
Supplemental Content	Access via ScholarShip
Subjects	Brucella abortus. Proteins. Manganese. Metals--Toxicology.

Summary	Divalent cationic metals are important trace nutrients for all bacteria because metals serve as cofactors for proteins involved in numerous cellular functions. Despite the fact that metals play an essential role in bacterial physiology, metals become harmful to bacteria if accumulated in excess. To prevent metal-induced toxicity, bacteria employ a variety of factors, such as metal-responsive transcriptional regulators and metal efflux proteins, that function to maintain proper intracellular metal homeostasis. The Brucella strains are Gram-negative, pathogenic bacteria that cause the severe febrile zoonotic disease, brucellosis, and these bacteria require the metal manganese for growth and during host infection. While the manganese acquisition protein, MntH, is essential for the capacity of Brucella spp. to maintain sufficient manganese nutrition, the mechanisms by which these bacteria prevent manganese toxicity have not been determined. The studies of this thesis were performed to identify how Brucella spp. cope with manganese toxicity and whether these factors are essential for Brucella virulence.
General note	Presented to the faculty of the Department of Biology
General note	Advisor: R. Martin Roop II
General note	Title from PDF t.p. (viewed October 15, 2019).
Dissertation note	M.S. East Carolina University 2019.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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The CDF-type metal efflux protein EmfA is essential for Brucella abortus 2308 resistance to manganese toxicity and is a critical virulence determinant in experimentally-infected mice / by Matthew J. Johnsrude.

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