Tools

LEADER 03450cam 2200517Ii 4500

001 on1113942219;

003 OCoLC;

005 20200115101912.0;

006 m o d ;

007 cr unu||||||||;

008 190827s2019 ncua obm 000 0 eng d;

035 a| (Sirsi) o1113942219;

035 a| (OCoLC)1113942219;

049 a| EREE;

090 a| R857.T55;

100 1 a| Myers, Victoria,e| author.=| ^A1387196;

245 10 a| Analysis of electrospun Tß4 coated scaffolds :b| a tissue engineering study /c| by Victoria Myers.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2019.;

300 a| 77 pages :b| color illustrations;

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 3.494 MB2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2019.;

500 a| Presented to the faculty of the Department of Engineering;

500 a| Advisor: Barbara Muller-Borer;

500 a| Title from PDF t.p. (viewed January 9, 2020).;

520 3 a| Tissue engineering is a field within regenerative medicine that promotes the regeneration/repair of damaged or diseased tissue and organs. This field focuses on three aspects: cells, growth factors, and scaffolds to achieve a successful implantable tissue/organ. Electrospun scaffolds are a common platform for tissue engineering applications. The objective of this project was to evaluate electrospun scaffolds' composition using poly (ethylene oxide) and ß- lactoglobulin with the incorporation of a wound healing protein, thymosin beta-4. The overall goal was to augment the biocompatibility characteristics of the scaffold, enhance the cellular microenvironment, and improve human mesenchymal stem cell cytocompatibility. Data was collected using multiple techniques such as goniometry and cell viability assays. The data was analyzed using either paired t-tests or analysis of variance (ANOVA). The analyzes compared treatments to determine Tß4's effect on cell proliferation and integration. Though the results did not reflect statistical significance, the results suggest that Tß4 did not promote cell proliferation and did not cause adverse reactions. Although Tß4 did promote cell integration with the scaffold's microenvironment. Successful growth and integration of cells on fabricated scaffolds are critical to the development and study of 3D tissue structures. Therefore, this approach has the potential to further research into alternative therapies for wound healing.;

504 a| Includes bibliographical references.;

650 0 a| Tissue engineering.=| ^A652471;

650 0 a| Tissue scaffolds.=| ^A1389273;

653 a| Thymosin-Beta-4;

653 a| Nanofiber;

653 a| Coating;

700 1 a| Muller-Borer, Barbara J.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Engineering.=| ^A1231592;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/7463;

949 o| wjh;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 5168886;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5168886-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 8/27/2019x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5168886-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 8/27/2019x| ETDz| HERESOURCE;

Analysis of electrospun Tß4 coated scaffolds : a tissue engineering study / by Victoria Myers.

Author/creator	Myers, Victoria author.
Other author	Muller-Borer, Barbara J., degree supervisor.
Other author	East Carolina University. Department of Engineering.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2019.
Description	77 pages : color illustrations
Supplemental Content	Access via ScholarShip
Subjects	Tissue engineering. Tissue scaffolds.

Summary	Tissue engineering is a field within regenerative medicine that promotes the regeneration/repair of damaged or diseased tissue and organs. This field focuses on three aspects: cells, growth factors, and scaffolds to achieve a successful implantable tissue/organ. Electrospun scaffolds are a common platform for tissue engineering applications. The objective of this project was to evaluate electrospun scaffolds' composition using poly (ethylene oxide) and ß- lactoglobulin with the incorporation of a wound healing protein, thymosin beta-4. The overall goal was to augment the biocompatibility characteristics of the scaffold, enhance the cellular microenvironment, and improve human mesenchymal stem cell cytocompatibility. Data was collected using multiple techniques such as goniometry and cell viability assays. The data was analyzed using either paired t-tests or analysis of variance (ANOVA). The analyzes compared treatments to determine Tß4's effect on cell proliferation and integration. Though the results did not reflect statistical significance, the results suggest that Tß4 did not promote cell proliferation and did not cause adverse reactions. Although Tß4 did promote cell integration with the scaffold's microenvironment. Successful growth and integration of cells on fabricated scaffolds are critical to the development and study of 3D tissue structures. Therefore, this approach has the potential to further research into alternative therapies for wound healing.
General note	Presented to the faculty of the Department of Engineering
General note	Advisor: Barbara Muller-Borer
General note	Title from PDF t.p. (viewed January 9, 2020).
Dissertation note	M.S. East Carolina University 2019.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

Availability

Library	Location	Call Number	Status	Item Actions
	Electronic Resources	Access Content Online	✔ Available

Analysis of electrospun Tß4 coated scaffolds : a tissue engineering study / by Victoria Myers.

Click here for more information about this title

Availability