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035 a| (Sirsi) o1144495084;

035 a| (OCoLC)1144495084;

049 a| EREE;

090 a| QP361;

100 1 a| Bunner, Wyatt,e| author.?| UNAUTHORIZED;

245 10 a| AgRP neuron activity is required for acute exercise-induced feeding /c| by Wyatt Bunner.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2019.;

300 a| 58 pages :b| illustrations (chiefly color);

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 1.501 MB2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2019.;

500 a| Presented to the faculty of the Department of Kinesiology;

500 a| Advisor: Hu Huang;

500 a| Title from PDF t.p. (viewed March 25, 2020).;

520 3 a| While much is known about the role of neuropeptide Y/agouti-regulated peptide (NPY/AgRP) and pro-opiomelanocortin (POMC) neurons to regulate energy homeostasis, little is known about how forced energy expenditure, such as exercise, modulates these neurons and how this relates to energy intake. Therefore, we investigated the effects of acute exercise on neuronal activity in the arcuate nucleus (ARC) of the hypothalamus. To accomplish this, we utilized immunohistochemistry and patch-clamp electrophysiology experiments on NPY-GFP transgenic mice immediately after an acute bout of treadmill exercise. Due to the ability of NPY/AgRP and POMC neurons to mediate energy homeostasis, food intake studies were also performed immediately after an acute bout of treadmill exercise. AgRP-Ires-cre transgenic mice were used to induce loss in AgRP neuronal activation by bilaterally injecting an inhibitory cre-recombinase-dependent Adeno Associated Virus (AAV-hM4Di-mCherry) to assess AgRP neurons in food intake post-exercise. While we observed no difference in activation in POMC neurons, immediately after exercise, activation in ARC NPY/AgRP neurons is significantly increased compared to the sedentary control group; further confirmed by electrophysiology recording showing a significant increase in firing rate in NPY/AgRP neurons after acute exercise. Food intake was significantly increased immediately after an acute bout of exercise. This exercise-induced food intake was abolished when AgRP neuron activation was inhibited. Neuronal inhibition of AgRP neurons had no effect of hypothalamic paraventricular nucleus (PVN) activation immediately after a bout of acute exercise. Our results demonstrate NPY/AgRP activation is critical for acute exercise induced food intake in mice, thus providing insight into the subtle exercise induced response to facilitate energy replacement.;

504 a| Includes bibliographical references.;

650 0 a| Neuronsx| Physiology.=| ^A3656;

650 0 a| Exercise tests.=| ^A2525;

650 0 a| Neuropeptide Y.=| ^A372343;

650 0 a| Hypothalamus.=| ^A87;

653 a| Arcuate Nucleus;

653 a| Neuronal Activation;

700 1 a| Huang, Hu,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Kinesiology.?| UNAUTHORIZED;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/7624;

949 o| wjh;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 5266579;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5266579-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 3/13/2020x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5266579-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 3/13/2020x| ETDz| HERESOURCE;

AgRP neuron activity is required for acute exercise-induced feeding / by Wyatt Bunner.

Author/creator	Bunner, Wyatt author.
Other author	Huang, Hu, degree supervisor.
Other author	East Carolina University. Department of Kinesiology.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2019.
Description	58 pages : illustrations (chiefly color)
Supplemental Content	Access via ScholarShip
Subjects	Neurons--Physiology. Exercise tests. Neuropeptide Y. Hypothalamus.

Summary	While much is known about the role of neuropeptide Y/agouti-regulated peptide (NPY/AgRP) and pro-opiomelanocortin (POMC) neurons to regulate energy homeostasis, little is known about how forced energy expenditure, such as exercise, modulates these neurons and how this relates to energy intake. Therefore, we investigated the effects of acute exercise on neuronal activity in the arcuate nucleus (ARC) of the hypothalamus. To accomplish this, we utilized immunohistochemistry and patch-clamp electrophysiology experiments on NPY-GFP transgenic mice immediately after an acute bout of treadmill exercise. Due to the ability of NPY/AgRP and POMC neurons to mediate energy homeostasis, food intake studies were also performed immediately after an acute bout of treadmill exercise. AgRP-Ires-cre transgenic mice were used to induce loss in AgRP neuronal activation by bilaterally injecting an inhibitory cre-recombinase-dependent Adeno Associated Virus (AAV-hM4Di-mCherry) to assess AgRP neurons in food intake post-exercise. While we observed no difference in activation in POMC neurons, immediately after exercise, activation in ARC NPY/AgRP neurons is significantly increased compared to the sedentary control group; further confirmed by electrophysiology recording showing a significant increase in firing rate in NPY/AgRP neurons after acute exercise. Food intake was significantly increased immediately after an acute bout of exercise. This exercise-induced food intake was abolished when AgRP neuron activation was inhibited. Neuronal inhibition of AgRP neurons had no effect of hypothalamic paraventricular nucleus (PVN) activation immediately after a bout of acute exercise. Our results demonstrate NPY/AgRP activation is critical for acute exercise induced food intake in mice, thus providing insight into the subtle exercise induced response to facilitate energy replacement.
General note	Presented to the faculty of the Department of Kinesiology
General note	Advisor: Hu Huang
General note	Title from PDF t.p. (viewed March 25, 2020).
Dissertation note	M.S. East Carolina University 2019.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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AgRP neuron activity is required for acute exercise-induced feeding / by Wyatt Bunner.

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