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035 a| (Sirsi) o30651301;

035 a| (OCoLC)30651301;

049 a| EREE;

050 4 a| QL666.C584b| O87 1993;

100 1 a| Osentoski, Matthew F.,e| author.?| UNAUTHORIZED;

245 10 a| MtDNA variation in the gopher tortoise, Gopherus polyphemus /c| by Matthew F. Osentoski.;

264 0 c| 1993.;

300 a| 48 pages, 26 unnumbered leaves :b| illustrations (some color), maps ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 1993;

500 a| Submitted to the faculty of the Department of Biology.;

500 a| Advisor: Trip Lamb;

520 3 a| The slow rate of mtDNA evolution in turtles poses a limitation on the levels of intraspecific variation detectable by conventional restriction fragment surveys. Here I explore an alternative assay for turtle evolutionary genetics, in which PCR-amplified regions of the mitochondrial genome are restricted using endonucleases with tetranucleotide recognition sites. The PCR-restriction approach was used to examine genetic variation in the gopher tortoise, Gopherus polyphemus. Restriction fragment polymorphisms, representing four amplified regions of the mitochondrial genome, were used to assess population genetic structure of the tortoise rangewide. Eight restriction endonucleases were used to survey 112 tortoise (55 localities), distinguishing a total of 36 mtDNA genotypes. Both UPGMA and parsimony analyses identified three major geographic assemblages; Eastern, Western, and Mid-Florida. The Eastern and Western assemblages bisect the species' range in the vicinity of the Apalachicola-Chattahoochee drainages, whereas the smaller Mid-Florida assemblage is associated largely with the Brooksville Ridge. The observed east-west break is roughly concordant with other distributional and genetic (nuclear and mitochondrial) studies that have been conducted in the Southeast. Both the Eastern/Western and Mid-Florida patterns may have been shaped by historic eustatic sea level fluctuation. Overall, compared to conventional methodology, the PCR-restriction approach offers promise for revealing intraspecific variation in turtles.;

504 a| Includes bibliographical references (leaves 41-48).;

650 0 a| Gopher tortoisex| Variation.=| ^A842535;

650 0 a| Mitochondrial DNA.=| ^A348042;

650 7 a| Mitochondrial DNA.2| fast0| (OCoLC)fst01024018;

655 7 a| dissertations.2| aat0| (CStmoGRI)aatgf300028029;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Lamb, Trip,e| degree supervisor.1| https://id.oclc.org/worldcat/entity/E39PCjJTcgXXcWKJDt7Rk73Qhd=| ^A1277551;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/12680;

949 a| Click on web addressw| asish| joyner101;

949 a| Click on web addressw| asish| hsl111;

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MtDNA variation in the gopher tortoise, Gopherus polyphemus / by Matthew F. Osentoski.

Author/creator	Osentoski, Matthew F. author.
Other author	Lamb, Trip, degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	1993.
Description	48 pages, 26 unnumbered leaves : illustrations (some color), maps ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Gopher tortoise--Variation. Mitochondrial DNA.

Summary	The slow rate of mtDNA evolution in turtles poses a limitation on the levels of intraspecific variation detectable by conventional restriction fragment surveys. Here I explore an alternative assay for turtle evolutionary genetics, in which PCR-amplified regions of the mitochondrial genome are restricted using endonucleases with tetranucleotide recognition sites. The PCR-restriction approach was used to examine genetic variation in the gopher tortoise, Gopherus polyphemus. Restriction fragment polymorphisms, representing four amplified regions of the mitochondrial genome, were used to assess population genetic structure of the tortoise rangewide. Eight restriction endonucleases were used to survey 112 tortoise (55 localities), distinguishing a total of 36 mtDNA genotypes. Both UPGMA and parsimony analyses identified three major geographic assemblages; Eastern, Western, and Mid-Florida. The Eastern and Western assemblages bisect the species' range in the vicinity of the Apalachicola-Chattahoochee drainages, whereas the smaller Mid-Florida assemblage is associated largely with the Brooksville Ridge. The observed east-west break is roughly concordant with other distributional and genetic (nuclear and mitochondrial) studies that have been conducted in the Southeast. Both the Eastern/Western and Mid-Florida patterns may have been shaped by historic eustatic sea level fluctuation. Overall, compared to conventional methodology, the PCR-restriction approach offers promise for revealing intraspecific variation in turtles.
General note	Submitted to the faculty of the Department of Biology.
General note	Advisor: Trip Lamb
Dissertation note	M.S. East Carolina University 1993
Bibliography note	Includes bibliographical references (leaves 41-48).
Genre/form	dissertations.
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

MtDNA variation in the gopher tortoise, Gopherus polyphemus / by Matthew F. Osentoski.

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