Biological small angle scattering theory and practice / Eaton E. Lattman, Thomas D. Grant, Edward H. Snell.
| Author/creator | Lattman, Eaton E., 1940- |
| Other author | Grant, Thomas D. (Staff scientist) |
| Other author | Snell, Edward H. |
| Format | Electronic |
| Edition | First edition. |
| Publication Info | Oxford, United Kingdom : Oxford University Press, 2018. |
| Description | xii, 268 pages : illustrations, charts ; 24 cm. |
| Supplemental Content | Full text available from Ebook Central - Academic Complete |
| Subjects |
| Series | IUCr monographs on crystallography ; 29 International Union of Crystallography monographs on crystallography ; 29. ^A371843 |
| Contents | Machine generated contents note: pt. 1 Introduction -- 1.Introduction -- pt. 2 Theory of Small Angle Scattering -- 2.Theoretical Background -- 2.1.Introduction -- 2.2.Scattering Basics -- 2.3.Multi-Scatterer Systems: The Debye Equation -- 2.4.The Pair Distance Distribution Function, P(r) -- 2.5.The Concept of Contrast in Solution Scattering -- 2.6.Resolution and Information Content -- 2.7.Summary -- 3.Quantities Directly Measurable by Scattering -- 3.1.Introduction -- 3.2.Invariants -- 3.3.Global Characteristics of the I(q) Curve -- 3.4.Comparison of Invariants -- 3.5.The Kratky Plot Distinguishes Globularity and Flexibility -- 3.6.Summary -- 4.Shape Reconstructions from Small Angle Scattering Data -- 4.1.Calculating Scattering Profiles from Three-Dimensional Models -- 4.2.Ab Initio Modeling -- 4.3.Flexible Fitting -- 4.4.Rigid Body Modeling -- 4.5.Docking Algorithms -- 4.6.Mixtures -- 4.7.Ensembles -- 4.8.Hybrid Modeling -- 4.9.Summary |
| Contents | Note continued: pt. 3 Practical Aspects of Small Angle Scattering -- 5.Before the Beamtime -- 5.1.Sample Production -- 5.2.Buffer Choice and Matching -- 5.3.Sample Optimization -- 5.4.Transport of the Sample -- 5.5.Practical Preparations before Data Collection -- 5.6.Quality Control Checks -- 5.7.Contrast Matching for X-Ray and Neutron Cases -- 5.8.Summary -- 6.Making the Best Use of Beamtime -- 6.1.Sample-to-Detector Distance -- 6.2.Instrument Calibration -- 6.3.Sample Concentration -- 6.4.Number of Exposures/Exposure Time -- 6.5.Image Integration -- 6.6.Buffer Collection and Subtraction -- 6.7.Initial Parameter Evaluation -- 6.8.The Guinier Plot -- 6.9.Estimating the Radius of Gyration, Rg -- 6.10.Calculating the Pair Distance Distribution Function, P(r) -- 6.11.Estimating Forward Scattering, I(0), and Maximum Dimension, Dmax -- 6.12.Assessing Flexibility -- 6.13.Estimating Volume and Molecular Weight -- 6.14.Evaluating Radiation Damage and Concentration Dependence |
| Contents | Note continued: 6.15.Assessing Accuracy of Buffer Subtraction -- 6.16.Summary -- 7.Examples of Data Collection and Processing -- 7.1.Sample -- 7.2.Data -- 7.3.Checking Buffer Blanks -- 7.4.Avoiding Radiation Damage -- 7.5.Qualitative Comparison of Concentrations -- 7.6.Using Guinier Plots To Detect Interparticle Interactions and Calculate Rg and I(0) -- 7.7.Calculating P(r) and Dmax -- 7.8.Assessing Flexibility -- 7.9.Checking for Accurate Buffer Subtraction -- 7.10.Zero Extrapolation -- 7.11.Extracting Structural Information -- 7.12.Summary -- 8.Instrumental and Experimental Considerations -- 8.1.Detector Considerations -- 8.2.Integration -- 8.3.Limitations on Minimum q Resolution -- 8.4.Desmearing -- 8.5.Signal-to-Noise Considerations -- 8.6.Zero Extrapolation -- 8.7.Summary -- 9.SAXS Instrumentation -- 9.1.Laboratory SAXS -- 9.2.Synchrotron SAXS -- 9.3.Free Electron Laser X-Ray Sources -- 9.4.Sample Handling -- 9.5.Detectors |
| Contents | Note continued: 9.6.Data Acquisition and Analysis Software -- 9.7.Instrumentation for Time-Resolved Studies -- 9.8.Summary -- 10.Distinct Instrumental Approaches to SAXS -- 10.1.SEC-SAXS -- 10.2.Time-Resolved SAXS -- 10.3.High-Throughput SAXS -- 10.4.Summary -- 11.SANS -- 11.1.X-Rays and Neutrons -- 11.2.Neutron Sources -- 11.3.The Technique of Contrast Variation -- 11.4.Applications of Neutron Scattering -- 11.5.SANS Beamlines -- 11.6.Summary -- pt. 4 Applications Past, Present, and Future -- 12.Examples of Biological Small Angle Scattering -- 12.1.The Use of Contrast Matching -- 12.2.Time-Resolved Studies at Synchrotrons and XFELs -- 12.3.SAXS Providing Information Missing with Other Structural Techniques -- 12.4.Putting Complexes in Context -- 12.5.Functional Changes -- 12.6.Protein-Ligand Interactions -- 12.7.Dynamical Systems -- 12.8.Systems with Limited or No Structural Information -- 12.9.Intrinsically Disordered Proteins -- 12.10.Summary |
| Contents | Note continued: 13.Developments on the Horizon -- 13.1.The Impact of XFELs -- 13.2.Working with Cryocooled Samples -- 13.3.Anomalous or Resonant Scattering in SAXS -- 13.4.Summary -- 14.Pushing the Envelope -- 14.1.Introduction -- 14.2.Retrieving Three-Dimensional Structure Factors from One-Dimensional Solution Scattering Data -- 14.3.Application to Real Data -- 14.4.Summary. |
| Bibliography note | Includes bibliographical references (pages 247-266) and index. |
| Access restriction | Available only to authorized users. |
| Technical details | Mode of access: World Wide Web |
| Issued in other form | Electronic version: Lattman, Eaton E. (Eaton Edward), 1940- Biological small angle scattering. First edition. Oxford, United Kingdom : Oxford University Press, 2018 9780191649899 |
| Genre/form | Electronic books. |
| LCCN | 2018933085 |
| ISBN | 9780199670871 (hardback) |
| ISBN | 0199670870 (hardback) |
| ISBN | (electronic book) |
| ISBN | (electronic book) |
Availability
| Library | Location | Call Number | Status | Item Actions |
|---|---|---|---|---|
| Electronic Resources | Access Content Online | ✔ Available |