The Polymerase chain reaction / Kary B. Mullis, François Ferré, Richard A. Gibbs, editors ; foreword by James D. Watson.

Other author Mullis, Kary
Other author Ferré, François.
Other author Gibbs, Richard (Richard A.)
Format Book
Publication InfoBoston : Birkhäuser, ©1994.
Descriptionxxii, 458 pages : illustrations ; 26 cm
Subjects
Polymerase chain reaction.
Polymerase Chain Reaction.

Contents Methodology -- Basic methodology -- Manipulation of DNA by PCR / Kenshi Hayashi -- Cloning PCR products / Michael A. Frohman -- Optimization of multiplex PCRs / Jeffrey S. Chamberlain and Joel R. Chamberlain -- Preparation of nucleic acids for archival material / Darryl Shibata -- PCR amplification of viral DNA and viral host cell mRNAs in situ / Janet Embretson ... [et al.] -- Quantitation -- Quantitative PCR: an overview / F. Ferre ... [et al.] -- Quantification of DNAs by the polymerase chain reaction using an internal control / Donald M. Coen -- RT-PCR and mRNA quantitation / Jamel Chelly and Axel Kahn -- Analysis of human T-cell repertoires by PCR / Dominic G. Spinella and James M. Robertson -- Nonisotopic detection -- Ultrasensitive nonradioactive detection of PCR reactions: an overview / Richard H. Tullis -- Fluorescent detection methods for PCR analysis / Michael J. Heller -- Enzyme-labeled oligonucleotides / Eugene Tu and Edward Jablonski -- Application of the hybridization protection assay (HPA) to PCR / Norman C. Nelson and Sherrol H. McDonough.
Contents (cont) Instrumentation -- PCR instrumentation: where do we stand? / Christian C. Oste -- Rapid cycle DNA amplification / Carl T. Wittwer, Gudrun B. Reed and Kirk M. Ririe -- Automating the PCR process / H.R. Garner -- Sequencing -- PCR and DNA sequencing / Bjorn Andersson and Richard A. Gibbs -- Phage promoter-based methods for sequencing and screening for mutations / Steve S. Sommer and Erica L. Vielhaber -- Capture PCR: an efficient method for walking along chromosomal DNA and cDNA / Andre Rosenthal, Matthias Platzer and D. Stephen Charnock-Jones -- Applications -- General applications -- In vitro evolution of functional nucleic acids: high-affinity RNA ligands of the HIV-1 rev protein / Craig Tuerk ... [et al.] -- Application of PCR to forensic science / Bruce Budowle ... [et al.] -- Recreating the past by PCR / Matthias Hoss, Oliva Handt and Svante Paabo -- Nonbiological applications / Gavin Dollinger -- Genetic analysis -- RT-PCR and gene expression / Didier Montarras ... [et al.].
Contents (cont) Fingerprinting using arbitrarily primed PCR: application to genetic mapping, population biology, epidemiology and detection of differentially expressed RNAs / John Welsh and Michael McClelland -- Genetics, plants and the polymerase chain reaction / Bruno W.S. Sobral and Rhonda J. Honeycutt -- Assessment of therapy effectiveness -- PCR assessment of the efficacy of therapy in Philadelphia chromosome-positive leukemias / Stephen P. Hunger and Michael L. Cleary -- Detection of minimal residual disease (MRD) in acute lymphoblastic leukemia using clone-specific probes directed against V(D)J junctional sequences / Luc d'Auriol and Francois Sigaux -- Assessment of therapy effectiveness: infectious disease / Salvatore J. Arrigon -- Gene therapy / Richard A. Morgan and W. French Anderson -- Diagnostics -- PCR and cancer diagnostics: detection and characterization of single point mutations in oncogenes and antioncogenes / Manuel Perucho -- Clinical applications of the polymerase chain reaction / Belinda J.F. Rossiter and C. Thomas Caskey.
Contents (cont) Infectious diseases / W. John Martin -- PCR and the world of business -- PCR in the marketplace / Ellen Daniell -- PCR and scientific invention: the trial of DuPont vs. Cetus / Kary B. Mullis.
Local noteLittle-296824--305131003236R
Bibliography noteIncludes bibliographical references and index.
LCCN 94162449
ISBN0817636072 (hb. : Boston)
ISBN0817637508 (pbk. : Boston)
ISBN3764336072 (hb.)
ISBN3764337508 (pbk.)

Availability

Library Location Call Number Status Item Actions
Joyner General Stacks QP606.D46 P653 1994 ✔ Available Place Hold