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035 a| (Sirsi) o1257305212;

035 a| (OCoLC)1257305212;

049 a| EREE;

090 a| QR398;

100 1 a| Mazzara, Jordan,e| author.?| UNAUTHORIZED;

245 10 a| Impact of Espirito Santo virus on dengue virus growth kinetics in vitro /c| by Jordan Mazzara.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2021.;

300 a| 52 pages :b| illustrations;

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 563.8 KB2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2021.;

500 a| Presented to the faculty of the Department of Health Education and Promotion.;

500 a| Advisor: Stephanie Richards;

500 a| Title from PDF t.p. (viewed October 20, 2021).;

520 3 a| With more than half the world's population living in areas at risk, vector-borne diseases have been, and continue to be, a major threat to public health. One of the most important arboviruses today is dengue virus (DENV) which impacts approximately 390 million individuals each year. Dengue virus is transmitted primarily by Aedes aegypti and Ae. albopictus mosquitoes, which are now prevalent in just about every continent across the globe due to urbanization and climate change. Co-circulation of vectors capable of transmitting various pathogens put humans at risk for contracting one or more arboviruses either simultaneously or sequentially. Espirito Santo virus (ESV) has been characterized as an insect-specific virus (i.e., should not replicate or have cytopathic effects in mammalian cells). Here, we investigated ESV growth kinetics in various mammalian cell lines and evaluated the interaction between ESV and DENV in coinfection. We hypothesized that ESV would interfere with DENV replication in vitro. We show that ESV replicated in human embryonic kidney (HEK) cells without the presence of DENV, indicating that the virus may not be insect-specific. We also found that ESV seemed to inhibit the growth of DENV in Vero cells. However, DENV seemed to inhibit ESV growth in all cell lines. Although our findings did not support our initial hypothesis, we expect our findings will lead to further studies evaluating ESV alone and ESV coinfection with other medically important arboviruses and under different initial infecting doses. This could further discoveries in combating the global burden of arboviral diseases.;

504 a| Includes bibliographical references.;

650 0 a| Dengue viruses.=| ^A1259831;

650 0 a| Mosquitoes as carriers of disease.=| ^A6807;

650 0 a| Arbovirus infections.=| ^A223975;

653 a| Espirito Santo Virus;

653 a| Coinfection;

653 a| Insect Specific Virus;

700 1 a| Richards, Stephanie L.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Health Education and Promotion.?| UNAUTHORIZED;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/9093;

949 o| wjh;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 5636944;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5636944-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 6/22/2021x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5636944-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 6/22/2021x| ETDz| HERESOURCE;

Impact of Espirito Santo virus on dengue virus growth kinetics in vitro / by Jordan Mazzara.

Author/creator	Mazzara, Jordan author.
Other author	Richards, Stephanie L., degree supervisor.
Other author	East Carolina University. Department of Health Education and Promotion.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2021.
Description	52 pages : illustrations
Supplemental Content	Access via ScholarShip
Subjects	Dengue viruses. Mosquitoes as carriers of disease. Arbovirus infections.

Summary	With more than half the world's population living in areas at risk, vector-borne diseases have been, and continue to be, a major threat to public health. One of the most important arboviruses today is dengue virus (DENV) which impacts approximately 390 million individuals each year. Dengue virus is transmitted primarily by Aedes aegypti and Ae. albopictus mosquitoes, which are now prevalent in just about every continent across the globe due to urbanization and climate change. Co-circulation of vectors capable of transmitting various pathogens put humans at risk for contracting one or more arboviruses either simultaneously or sequentially. Espirito Santo virus (ESV) has been characterized as an insect-specific virus (i.e., should not replicate or have cytopathic effects in mammalian cells). Here, we investigated ESV growth kinetics in various mammalian cell lines and evaluated the interaction between ESV and DENV in coinfection. We hypothesized that ESV would interfere with DENV replication in vitro. We show that ESV replicated in human embryonic kidney (HEK) cells without the presence of DENV, indicating that the virus may not be insect-specific. We also found that ESV seemed to inhibit the growth of DENV in Vero cells. However, DENV seemed to inhibit ESV growth in all cell lines. Although our findings did not support our initial hypothesis, we expect our findings will lead to further studies evaluating ESV alone and ESV coinfection with other medically important arboviruses and under different initial infecting doses. This could further discoveries in combating the global burden of arboviral diseases.
General note	Presented to the faculty of the Department of Health Education and Promotion.
General note	Advisor: Stephanie Richards
General note	Title from PDF t.p. (viewed October 20, 2021).
Dissertation note	M.S. East Carolina University 2021.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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Impact of Espirito Santo virus on dengue virus growth kinetics in vitro / by Jordan Mazzara.

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