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003 OCoLC;

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008 210914s2021 ncua obm 000 0 eng d;

035 a| (Sirsi) o1268150121;

035 a| (OCoLC)1268150121;

049 a| EREE;

090 a| QP552.T68;

100 1 a| Pratt, Isaac,e| author.=| ^A736599;

245 10 a| Genome wide identification of ARF transcription factor gene family and their expression analysis in sweet potato /c| by Isaac Pratt.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2021.;

300 a| 1 online resource (37 pages) :b| illustrations (chiefly color);

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text file;

347 b| PDF;

347 c| 564.5 KB;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2021;

500 a| Presented to the faculty of the Department of Biology;

500 a| Advisor: Baohong Zhang;

500 a| Title from PDF t.p. (viewed July 12, 2022).;

520 3 a| Auxin response factors (ARFs) are a family of transcription factors that play an important role of auxin regulation through their binding with auxin response elements. ARF genes are represented by a large multigene family in plants; however, to our knowledge, the ARF gene family has not been well studied and characterized in sweet potatoes. In this study, a total of 26 possible ItfARF genes were identified in Ipomea trifida; however, ItfARF1a and ItfARF2a were found to be a single gene, resulting in a total of 25 ItfARF genes. Firstly, a comprehensive bioinformatics analysis of the ItfARF gene family was given, including conserved motifs, chromosomal locations, phylogenetic relationships, and a protein data breakdown. Furthermore, the expression patterns of ItfARF genes were analyzed within the storage roots and normal roots, at an early stage of development. ItfARF16b [and] ItfARF16c were both highly expressed in the storage root, with minimal to no expression in the normal root. ItfARF6a and ItfARF10a exhibited higher expression in the normal root, but not in the storage root. Subsequently, ItfARF1a, ItfARF2b, ItfARF3a, ItfARF6b, ItfARF8a, ItfARF8b and ItfARF10b were expressed in both root types with moderate to high expression for each. All ten of these ARF genes and their prominent expression signify their importance within the development of each respective root type. This study provides comprehensive information regarding the ARF family in sweet potatoes, which will be useful for future research to discover further functional verification of these ItfARF genes.;

504 a| Includes bibliographical references.;

650 0 a| Transcription factors.=| ^A680413;

650 0 a| Sweet potatoesx| Genetics.=| ^A1439583;

650 0 a| Roots (Botany)x| Development.=| ^A1439619;

653 a| auxin response factor;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

655 2 a| Academic Dissertation.0| (DNLM)D019478?| UNAUTHORIZED;

700 1 a| Zhang, Baohong,e| degree supervisor.=| ^A1031945;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 u| http://hdl.handle.net/10342/9371z| Access via ScholarShip;

949 o| wjh;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 5668813;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5668813-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 9/14/2021x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5668813-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 9/14/2021x| ETDz| HERESOURCE;

Genome wide identification of ARF transcription factor gene family and their expression analysis in sweet potato / by Isaac Pratt.

Author/creator	Pratt, Isaac author.
Other author	Zhang, Baohong, degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2021.
Description	1 online resource (37 pages) : illustrations (chiefly color)
Supplemental Content	Access via ScholarShip
Subjects	Transcription factors. Sweet potatoes--Genetics. Roots (Botany)--Development.

Summary	Auxin response factors (ARFs) are a family of transcription factors that play an important role of auxin regulation through their binding with auxin response elements. ARF genes are represented by a large multigene family in plants; however, to our knowledge, the ARF gene family has not been well studied and characterized in sweet potatoes. In this study, a total of 26 possible ItfARF genes were identified in Ipomea trifida; however, ItfARF1a and ItfARF2a were found to be a single gene, resulting in a total of 25 ItfARF genes. Firstly, a comprehensive bioinformatics analysis of the ItfARF gene family was given, including conserved motifs, chromosomal locations, phylogenetic relationships, and a protein data breakdown. Furthermore, the expression patterns of ItfARF genes were analyzed within the storage roots and normal roots, at an early stage of development. ItfARF16b [and] ItfARF16c were both highly expressed in the storage root, with minimal to no expression in the normal root. ItfARF6a and ItfARF10a exhibited higher expression in the normal root, but not in the storage root. Subsequently, ItfARF1a, ItfARF2b, ItfARF3a, ItfARF6b, ItfARF8a, ItfARF8b and ItfARF10b were expressed in both root types with moderate to high expression for each. All ten of these ARF genes and their prominent expression signify their importance within the development of each respective root type. This study provides comprehensive information regarding the ARF family in sweet potatoes, which will be useful for future research to discover further functional verification of these ItfARF genes.
General note	Presented to the faculty of the Department of Biology
General note	Advisor: Baohong Zhang
General note	Title from PDF t.p. (viewed July 12, 2022).
Dissertation note	M.S. East Carolina University 2021
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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Genome wide identification of ARF transcription factor gene family and their expression analysis in sweet potato / by Isaac Pratt.

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