Tools

LEADER 04075cam 2200661Ii 4500

001 on1268150273;

003 OCoLC;

005 20220726155329.0;

006 m o d ;

007 cr unu||||||||;

008 210914s2021 ncua obm 000 0 eng d;

035 a| (Sirsi) o1268150273;

035 a| (OCoLC)1268150273;

049 a| EREE;

100 1 a| Papariello, Alexis Rose,e| author.?| UNAUTHORIZED;

245 14 a| The endocannabinoid system regulates synaptogenesis in a cortical spheroid model of fetal neurodevelopment /c| by Alexis Rose Papariello.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2021.;

300 a| 1 online resource (152 pages) :b| illustrations (chiefly color).;

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text file;

347 b| PDF;

347 c| 3.716 MB;

490 1 a| ECU Brody School of Medicine dissertation;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| Ph. D.c| East Carolina Universityd| 2021;

500 a| Presented to the faculty of the Department of Pharmacology and Toxicology;

500 a| Advisor: David Taylor;

500 a| Advisor: Karen Litwa;

500 a| Advisor: Ken Soderstrom;

500 a| Title from PDF t.p. (viewed July 6, 2022).;

520 3 a| The endocannabinoid system plays a complex role in synaptogenesis and the subsequent development of neural circuitry during fetal brain development. The cannabinoid receptor type 1 controls synaptic strength at both excitatory and inhibitory synapses and thus contributes to the balance of excitatory and inhibitory signaling. Imbalances in the ratio of excitatory to inhibitory synapses have been implicated in various neuropsychiatric disorders associated with dysregulated central nervous system development including autism spectrum disorder, epilepsy, and schizophrenia. The role of the endocannabinoid system in human brain development has been difficult to study but advances in induced pluripotent stem cell technology have allowed us to model the fetal brain environment. Our cortical spheroid model resembles the cortex of the dorsal telencephalon during mid-fetal gestation and possesses functional synapses and spontaneous activity. Using SR141716A, we observed an increase in excitatory, and to a lesser extent, inhibitory synaptogenesis as measured by confocal image analysis. We also observed increased variability of neural activity and decreased GTPase RhoA activity. Overall, we have established that cortical spheroids express ECS components and are thus a useful model for exploring endocannabinoid mediation of childhood neuropsychiatric disease.;

504 a| Includes bibliographical references.;

650 2 a| Endocannabinoids.=| ^A1012986;

650 2 a| Synapses.=| ^A948337;

650 2 a| Cerebral Cortex.=| ^A921006;

655 2 a| Academic Dissertation.0| (DNLM)D019478?| UNAUTHORIZED;

653 a| cortical spheroid;

653 a| CB1;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Taylor, David A.,e| degree supervisor.=| ^A136235;

700 1 a| Litwa, Karen,e| degree supervisor.?| UNAUTHORIZED;

700 1 a| Soderstrom, Ken,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Pharmacology and Toxicology.?| UNAUTHORIZED;

830 0 a| ECU Brody School of Medicine dissertation.?| UNAUTHORIZED;

856 40 u| http://hdl.handle.net/10342/9390z| Access via ScholarShip;

949 o| wjh;

994 a| C0b| ERE;

096 a| QV 126;

596 a| 1 4;

998 a| 5668824;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5668824-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 9/14/2021x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5668824-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 9/14/2021x| ETDz| HERESOURCE;

The endocannabinoid system regulates synaptogenesis in a cortical spheroid model of fetal neurodevelopment / by Alexis Rose Papariello.

Author/creator	Papariello, Alexis Rose author.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2021.
Description	1 online resource (152 pages) : illustrations (chiefly color).
Supplemental Content	Access via ScholarShip
Subjects	Endocannabinoids. Synapses. Cerebral Cortex.

Other author/creator	Taylor, David A., degree supervisor.
Other author/creator	Litwa, Karen, degree supervisor.
Other author/creator	Soderstrom, Ken, degree supervisor.
Other author/creator	East Carolina University. Department of Pharmacology and Toxicology.
Series	ECU Brody School of Medicine dissertation ECU Brody School of Medicine dissertation. UNAUTHORIZED
Summary	The endocannabinoid system plays a complex role in synaptogenesis and the subsequent development of neural circuitry during fetal brain development. The cannabinoid receptor type 1 controls synaptic strength at both excitatory and inhibitory synapses and thus contributes to the balance of excitatory and inhibitory signaling. Imbalances in the ratio of excitatory to inhibitory synapses have been implicated in various neuropsychiatric disorders associated with dysregulated central nervous system development including autism spectrum disorder, epilepsy, and schizophrenia. The role of the endocannabinoid system in human brain development has been difficult to study but advances in induced pluripotent stem cell technology have allowed us to model the fetal brain environment. Our cortical spheroid model resembles the cortex of the dorsal telencephalon during mid-fetal gestation and possesses functional synapses and spontaneous activity. Using SR141716A, we observed an increase in excitatory, and to a lesser extent, inhibitory synaptogenesis as measured by confocal image analysis. We also observed increased variability of neural activity and decreased GTPase RhoA activity. Overall, we have established that cortical spheroids express ECS components and are thus a useful model for exploring endocannabinoid mediation of childhood neuropsychiatric disease.
General note	Presented to the faculty of the Department of Pharmacology and Toxicology
General note	Advisor: David Taylor
General note	Advisor: Karen Litwa
General note	Advisor: Ken Soderstrom
General note	Title from PDF t.p. (viewed July 6, 2022).
Dissertation note	Ph. D. East Carolina University 2021
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

Availability

Library	Location	Call Number	Status	Item Actions
	Electronic Resources	Access Content Online	✔ Available

The endocannabinoid system regulates synaptogenesis in a cortical spheroid model of fetal neurodevelopment / by Alexis Rose Papariello.

Click here for more information about this title

Availability