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245 00 a| Immunochemical protocolsh| [electronic resource] /c| edited by Robert Burns.;

250 a| 3rd ed.;

260 a| Totowa, N.J. :b| Humana Press,;

300 a| viii, 317 p. :b| ill. ;c| 24 cm.;

490 1 a| Methods in molecular biology ;v| 295;

504 a| Includes bibliographical references and index.;

505 0 a| Immunization strategies for antibody production / Robert Burns -- Synthetic peptides as antigens for antibody production / David C. Hancock and Nicola J. O'Reilly -- Production of polyclonal antibody in rabbits / David C. Hancock and Nicola J. O'Reilly -- Making hybridomas / Robert Burns -- Growing hybridomas / Gary Entrican and Gareth Young -- Phage display vectors for the in vitro generation of human antibody fragments / Michael Hust and Stefan Dobel -- Purification of antibodies and preparation of antibodies fragments / Graham D. Kemp -- Conjugation of antibodies to alkaline phosphatase / G. Brian Wisdom -- Conjugation of antibodies to horseradish peroxidase / G. Brian Wisdom -- Conjugation of antibodies to fluorescein or rhodamine / G. Brian Wisdom -- Conjugation of antibodies to biotin / James D. Hirsch and Rosaria P. Haughland -- Preparation of colloidal gold probes / David Hughes -- Immunogold probes in light microscopy / David Hughes -- Immunoelectron microscope techniques in plant virus diagnosis / Daphne M. Wright -- Dual-antibody sandwich enzyme-linked immunosorbent assay / William Jordan -- Competitive enzyme-linked immunosorbent assay / William Jordan -- Immunoblotting techniques / Barbara Magi and Sabrina Liberatori -- Epitope mapping / Glenn E. Morris -- Generation and testing of immunoaffinity columns / Weilin L. Shelver -- Immunocapture-polymerase chain reaction / Vincent Mulholland -- Magnetic cell sorting / Maciej Zborowski and Jeffrey J. Chalmers -- Cell enrichment and immunochemical staining / Oscar R. Lara and Jeffrey J. Chalmers.;

506 a| Available only to authorized users.;

538 a| Mode of access: World Wide Web;

650 0 a| Immunochemistryv| Laboratory manuals.=| ^A3595;

650 12 a| Antibodiesx| isolation & purificationv| Laboratory Manuals.=| ^A1201901;

650 12 a| Immunochemistryx| methodsv| Laboratory Manuals.=| ^A1203011;

655 0 a| Electronic books.=| ^A491897;

700 1 a| Burns, Robert,d| 1959-=| ^A942222;

830 0 a| Methods in molecular biology (Clifton, N.J.) ;v| v. 295.=| ^A682607;

856 40 z| Full text available from eBooks on EBSCOhostu| https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=131670&scope=site&custid=s5822723&authtype=ip,shib;

949 a| CLICK ON WEB ADDRESSw| ASISh| JOYNER188;

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596 a| 1 3 4;

998 a| 5691918;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5691918-1001l| JNETm| JOYNERr| Ys| Yt| JNESSBKu| 9/18/2021x| EBOOKz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5691918-2001l| HSLELECm| HSLr| Ys| Yt| HEBKu| 9/18/2021x| EBOOKz| HERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5691918-3001l| MNETm| JMUSICr| Ys| Yt| MNESSBKu| 9/18/2021x| EBOOKz| JERESOURCE;

Immunochemical protocols / edited by Robert Burns.

Other author	Burns, Robert, 1959-
Format	Electronic
Edition	3rd ed.
Publication Info	Totowa, N.J. : Humana Press,
Description	viii, 317 p. : ill. ; 24 cm.
Supplemental Content	Full text available from eBooks on EBSCOhost
Subjects	Immunochemistry--Laboratory manuals. Antibodies--isolation & purification. --Laboratory Manuals. Immunochemistry--methods. --Laboratory Manuals.

Series	Methods in molecular biology ; 295 Methods in molecular biology (Clifton, N.J.) ; v. 295. ^A682607
Contents	Immunization strategies for antibody production / Robert Burns -- Synthetic peptides as antigens for antibody production / David C. Hancock and Nicola J. O'Reilly -- Production of polyclonal antibody in rabbits / David C. Hancock and Nicola J. O'Reilly -- Making hybridomas / Robert Burns -- Growing hybridomas / Gary Entrican and Gareth Young -- Phage display vectors for the in vitro generation of human antibody fragments / Michael Hust and Stefan Dobel -- Purification of antibodies and preparation of antibodies fragments / Graham D. Kemp -- Conjugation of antibodies to alkaline phosphatase / G. Brian Wisdom -- Conjugation of antibodies to horseradish peroxidase / G. Brian Wisdom -- Conjugation of antibodies to fluorescein or rhodamine / G. Brian Wisdom -- Conjugation of antibodies to biotin / James D. Hirsch and Rosaria P. Haughland -- Preparation of colloidal gold probes / David Hughes -- Immunogold probes in light microscopy / David Hughes -- Immunoelectron microscope techniques in plant virus diagnosis / Daphne M. Wright -- Dual-antibody sandwich enzyme-linked immunosorbent assay / William Jordan -- Competitive enzyme-linked immunosorbent assay / William Jordan -- Immunoblotting techniques / Barbara Magi and Sabrina Liberatori -- Epitope mapping / Glenn E. Morris -- Generation and testing of immunoaffinity columns / Weilin L. Shelver -- Immunocapture-polymerase chain reaction / Vincent Mulholland -- Magnetic cell sorting / Maciej Zborowski and Jeffrey J. Chalmers -- Cell enrichment and immunochemical staining / Oscar R. Lara and Jeffrey J. Chalmers.
Bibliography note	Includes bibliographical references and index.
Access restriction	Available only to authorized users.
Technical details	Mode of access: World Wide Web
Genre/form	Electronic books.
LCCN	2004009405
ISBN	1588292746 (alk. paper)

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