Tools

LEADER 04280ctm 2200541 i 4500

001 ocm32424562;

003 OCoLC;

005 20240729154348.0;

008 950505s1994 xx a bm 000 0 eng d;

035 a| (Sirsi) o32424562;

035 a| (OCoLC)32424562;

049 a| EREE;

050 4 a| QL638.P358b| H37 1994;

100 1 a| Hardee, Jennifer J.,e| author.?| UNAUTHORIZED;

245 10 a| Major histocompatibility complex class II A gene polymorphism in the striped bass, Morone saxatilis /c| by Jennifer J. Hardee.;

264 0 c| 1994.;

300 a| 53 leaves :b| illustrations ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 1994;

500 a| Submitted to the faculty of the Department of Biology.;

500 a| Advisor: Thomas J. McConnell;

520 3 a| Polymerase chain reaction (PCR) technology was implemented in the amplification of the major histocompatibility complex (MHC) class II A genes in striped bass {Morone saxatilis). Several PCR reactions were performed on RNA from a Roanoke River striped bass and from a Santee Cooper striped bass. After cloning and sequencing of the PCR products, the sequences were analyzed and compared to the MHC class II A genes of humans, zebrafish, and the nurse shark. This comparison revealed that the PCR products were representative of the entire coding region of the MHC class II A genes. Four different class II A sequences were identified in the Santee Cooper striped bass and three different sequences were identified in the Roanoke River striped bass. These genes all exhibit concentrated variability within the al domain which is the domain involved in forming the peptide binding cleft. The number of different class II A sequences identified in each fish suggests the presence of multiple A gene loci. This observation is supported by Southern blot analysis of genomic DNA from other fish of these same populations. The Roanoke River fish, which are thought to be anadromous, have at least two and maybe as many as four A loci. The Santee Cooper fish which are from a land locked reservoir system are less polymorphic than the Roanoke River fish, however, there is evidence of at least two A loci. Although the striped bass class II A genes are only about 30% similar to mammalian class II A genes, the overall gene organization appears to be conserved among vertebrates. There are four exons that encode for the leader peptide, al domain, al domain, connecting peptide, transmembrane region, and the cytoplasmic tail. The striped bass A sequences are about 50% similar to the zebrafish sequences and 30% similar to the shark.;

504 a| Includes bibliographical references (leaves 50-53).;

650 0 a| Striped bass.=| ^A69980;

650 0 a| Major histocompatibility complex.=| ^A13757;

650 0 a| Fishesx| Immunology.=| ^A285730;

650 2 a| Major Histocompatibility Complex.0| (DNLM)D008285=| ^A950182;

655 2 a| Academic Dissertation.0| (DNLM)D019478?| UNAUTHORIZED;

650 7 a| Fishesx| Immunology.2| fast0| (OCoLC)fst00926518;

650 7 a| Major histocompatibility complex.2| fast0| (OCoLC)fst01006236;

650 7 a| Striped bass.2| fast0| (OCoLC)fst01135489;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| McConnell, Thomas J.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/12021;

949 a| Click on web addressw| asish| joyner101;

949 a| Click on web addressw| asish| hsl111;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 574613;

999 a| ASK AT SPECIAL COLLECTIONS DESKw| ASISc| 1i| 1007810d| 1/24/2002l| JSCJTHDm| JOYNERr| Ys| Yt| JSCJTHDu| 1/24/2002x| ETDz| JSPECCOLLo| .STAFF. 0;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 574613-3001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 7/1/2024x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 574613-4001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 7/1/2024x| ETDz| HERESOURCE;

Major histocompatibility complex class II A gene polymorphism in the striped bass, Morone saxatilis / by Jennifer J. Hardee.

Author/creator	Hardee, Jennifer J. author.
Other author	McConnell, Thomas J., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	1994.
Description	53 leaves : illustrations ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Striped bass. Major histocompatibility complex. Fishes--Immunology. Major Histocompatibility Complex.

Summary	Polymerase chain reaction (PCR) technology was implemented in the amplification of the major histocompatibility complex (MHC) class II A genes in striped bass {Morone saxatilis). Several PCR reactions were performed on RNA from a Roanoke River striped bass and from a Santee Cooper striped bass. After cloning and sequencing of the PCR products, the sequences were analyzed and compared to the MHC class II A genes of humans, zebrafish, and the nurse shark. This comparison revealed that the PCR products were representative of the entire coding region of the MHC class II A genes. Four different class II A sequences were identified in the Santee Cooper striped bass and three different sequences were identified in the Roanoke River striped bass. These genes all exhibit concentrated variability within the al domain which is the domain involved in forming the peptide binding cleft. The number of different class II A sequences identified in each fish suggests the presence of multiple A gene loci. This observation is supported by Southern blot analysis of genomic DNA from other fish of these same populations. The Roanoke River fish, which are thought to be anadromous, have at least two and maybe as many as four A loci. The Santee Cooper fish which are from a land locked reservoir system are less polymorphic than the Roanoke River fish, however, there is evidence of at least two A loci. Although the striped bass class II A genes are only about 30% similar to mammalian class II A genes, the overall gene organization appears to be conserved among vertebrates. There are four exons that encode for the leader peptide, al domain, al domain, connecting peptide, transmembrane region, and the cytoplasmic tail. The striped bass A sequences are about 50% similar to the zebrafish sequences and 30% similar to the shark.
General note	Submitted to the faculty of the Department of Biology.
General note	Advisor: Thomas J. McConnell
Dissertation note	M.S. East Carolina University 1994
Bibliography note	Includes bibliographical references (leaves 50-53).
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

Availability

Library	Location	Call Number	Status	Item Actions
Joyner	University Archives	ASK AT SPECIAL COLLECTIONS DESK	✔ Available	Request Material
	Electronic Resources	Access Content Online	✔ Available

Major histocompatibility complex class II A gene polymorphism in the striped bass, Morone saxatilis / by Jennifer J. Hardee.

Click here for more information about this title

Availability