A study of nucleolar organizing region polymorphism in Bufo terrestris / by Thomas D. Lupton.
| Author/creator | Lupton, Thomas D. author. |
| Other author | Wiley, John E., degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 1994. |
| Description | iv, 35 leaves, 2 unnumbered leaves of plates : illustrations ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | Nucleolar organizing regions are polymorphic in their locations in the chromosomes of Bufo terrestris. Different populations were studied to determine the extent and the types of rDNA polymorphism and to gain some insight into the causes of the phenomenon. This study found polymorphisms occurring in 2 of the 16 specimens examined from Pamlico County and none of the 38 specimens from Dare County. The polymorphic sites were found on chromosomes 2, 5, and 8. Fluorescent in situ hybridization using XIr11, a Xenopus laevis rDNA probe that localizes rDNA, showed the same results as NOR silver nitrate staining. Also, a 16 basepair inverted repeat (5'-GGGGCACATTTACTTA ... TAAGTAAATCTGCCCC-3') found around a Vi transposable element in X. laevis was not found in B. terrestris using fluorescent in situ hybridization and Southern blotting techniques. Therefore, neither the Vi element nor a transposable element with the same inverted repeat as the Vi element appears to be responsible for the movement of the NORs in B. terrestr̕s. |
| General note | Submitted to the faculty of the Department of Biology. |
| General note | Advisor: John E. Wiley |
| Dissertation note | M.S. East Carolina University 1994 |
| Bibliography note | Includes bibliographical references (leaves 24-26). |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |