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035 a| (OCoLC)1344380244z| (OCoLC)1371248715;

049 a| EREE;

100 1 a| Kirsanov, Oleksandr,e| author.?| UNAUTHORIZED;

245 10 a| Investigating mechanisms regulating mammalian spermatogonial differentiation and meiotic initiation /c| by Oleksandr Kirsanov.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2022.;

300 a| 1 online resource (223 pages) :b| illustrations (chiefly color).;

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text file;

347 b| PDF;

347 c| 5.911 MB;

490 1 a| ECU Brody of Medicine dissertation;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| Ph. D.c| East Carolina Universityd| 2022;

500 a| Presented to the Faculty of the Department of Anatomy & Cell Biology;

500 a| Advisor: Christopher B. Geyer;

500 a| Title from PDF t.p. (viewed February 6, 2024).;

520 3 a| Spermatogonia differentiation and meiotic initiation are complex processes essential for male fertility. Despite knowing for nearly 60 years that retinoic acid (RA) is instrumental for successful production of functional sperm, molecular mediators that regulate spermatogonial differentiation and prepare male germ cells for meiosis are poorly defined. The work presented here is organized into chapters and demonstrates my efforts to develop new tools to study germ cell development and to employ these tools to investigate the role and the developmental timeframes of RA activity during differentiation and meiotic initiation. In chapter 1, I examine published studies on development of germ cells starting at establishment of male germline in fetus to production of functional sperm in adult and highlight significant gaps in knowledge that are addressed with my work. In chapter 2, I describe negative effects of Everolimus, a pharmaceutical compound commonly used to treat cancer, on male fertility in mice. In chapter 3, I identify a new fluorescent reporter mouse model used to isolate large, highly enriched, precise population of germ cell at specific stages of their development and present a new culture system to study male germ cells in vitro. In chapter 4, I test the requirement for RA signaling during spermatogenesis.;

504 a| Includes bibliographical references.;

650 2 a| Spermatogonia.=| ^A1374356;

650 2 a| Spermatogenesis.=| ^A919033;

650 2 a| Animals.=| ^A919771;

653 a| Retinoic Acid;

653 a| Differentiation;

653 a| Meiosis;

653 a| Germ Cells;

653 a| Male Contraception;

655 7 a| dissertations.2| aat0| (CStmoGRI)aatgf300028029;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

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655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Geyer, Christopher B.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Anatomy and Cell Biology.?| UNAUTHORIZED;

830 0 a| ECU Brody of Medicine dissertation.?| UNAUTHORIZED;

856 40 u| http://hdl.handle.net/10342/11125z| Access via ScholarShip;

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999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5909010-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 9/14/2022x| ETDz| HERESOURCE;

Investigating mechanisms regulating mammalian spermatogonial differentiation and meiotic initiation / by Oleksandr Kirsanov.

Author/creator	Kirsanov, Oleksandr author.
Other author	Geyer, Christopher B., degree supervisor.
Other author	East Carolina University. Department of Anatomy and Cell Biology.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2022.
Description	1 online resource (223 pages) : illustrations (chiefly color).
Supplemental Content	Access via ScholarShip
Subjects	Spermatogonia. Spermatogenesis. Animals.

Series	ECU Brody of Medicine dissertation ECU Brody of Medicine dissertation. UNAUTHORIZED
Summary	Spermatogonia differentiation and meiotic initiation are complex processes essential for male fertility. Despite knowing for nearly 60 years that retinoic acid (RA) is instrumental for successful production of functional sperm, molecular mediators that regulate spermatogonial differentiation and prepare male germ cells for meiosis are poorly defined. The work presented here is organized into chapters and demonstrates my efforts to develop new tools to study germ cell development and to employ these tools to investigate the role and the developmental timeframes of RA activity during differentiation and meiotic initiation. In chapter 1, I examine published studies on development of germ cells starting at establishment of male germline in fetus to production of functional sperm in adult and highlight significant gaps in knowledge that are addressed with my work. In chapter 2, I describe negative effects of Everolimus, a pharmaceutical compound commonly used to treat cancer, on male fertility in mice. In chapter 3, I identify a new fluorescent reporter mouse model used to isolate large, highly enriched, precise population of germ cell at specific stages of their development and present a new culture system to study male germ cells in vitro. In chapter 4, I test the requirement for RA signaling during spermatogenesis.
General note	Presented to the Faculty of the Department of Anatomy & Cell Biology
General note	Advisor: Christopher B. Geyer
General note	Title from PDF t.p. (viewed February 6, 2024).
Dissertation note	Ph. D. East Carolina University 2022
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.
Genre/form	dissertations.
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

Investigating mechanisms regulating mammalian spermatogonial differentiation and meiotic initiation / by Oleksandr Kirsanov.

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