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008 230227s2022 ncua obm 000 0 eng d;

035 a| (Sirsi) o1371248866;

035 a| (OCoLC)1371248866;

049 a| EREE;

090 a| QK898.P4;

100 1 a| Maynard, Daniel Jarrell,e| author.?| UNAUTHORIZED;

245 10 a| Characterizing the role of pectin in cell wall composition and organ initiation in maize /c| by Daniel Jarrell Maynard.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2022.;

300 a| 1 online resource (72 pages) :b| illustrations (chiefly color);

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 2.664 MB2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2022.;

500 a| Presented to the Faculty of the Biology Department;

500 a| Advisor: Beth Thompson;

500 a| Title from PDF t.p. (viewed May 13, 2024).;

520 3 a| Plants are constantly developing new organs throughout their life and these organs arise from stem cell pools called meristems. Organ primordia are generated on the flanks of meristems when stem cells differentiate into new cell types. Newly differentiated cells are unable to move freely because they are surrounded by a rigid cell wall. To allow for proper organ outgrowth, the cell wall must be modified to allow for cell expansion. Organ initiation is closely linked to, and regulated by, distributions of the plant hormone auxin. The cell wall polysaccharide, pectin, is modified at the sites of auxin accumulation and is required for organ outgrowth, at least in some plants. Previous work in maize showed that floral meristems have differing localization of pectin that may coincide with differences in organ fate. We characterized meristems in the female inflorescence which give rise to different structures and different number of primordia. Mutants with altered floral meristem determinacy were assessed to determine if changes in floral meristem activity correlated with changes in pectin localization. The shoot apical meristem was assessed, and we found that pectin localization is not uniform between vegetative meristems and floral meristems. We found that meristems of the inflorescence localize pectin differently and that pectin modification is necessary for some organ development. Mutants that lose determinacy localize pectin differently which suggests that pectin composition in the cell wall is intimately linked to proper meristem activity and floral organ primordia formation.;

504 a| Includes bibliographical references.;

650 0 a| Pectin.=| ^A1471525;

650 0 a| Meristems.=| ^A826579;

650 0 a| Auxinx| Molecular aspects.=| ^A1380178;

650 0 a| Corn.=| ^A51307;

650 0 a| Plant cell walls.=| ^A11914;

700 1 a| Thompson, Beth E.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/12278;

949 o| wjh;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 5961438;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5961438-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 3/1/2023x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 5961438-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 3/1/2023x| ETDz| HERESOURCE;

Characterizing the role of pectin in cell wall composition and organ initiation in maize / by Daniel Jarrell Maynard.

Author/creator	Maynard, Daniel Jarrell author.
Other author	Thompson, Beth E., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2022.
Description	1 online resource (72 pages) : illustrations (chiefly color)
Supplemental Content	Access via ScholarShip
Subjects	Pectin. Meristems. Auxin--Molecular aspects. Corn. Plant cell walls.

Summary	Plants are constantly developing new organs throughout their life and these organs arise from stem cell pools called meristems. Organ primordia are generated on the flanks of meristems when stem cells differentiate into new cell types. Newly differentiated cells are unable to move freely because they are surrounded by a rigid cell wall. To allow for proper organ outgrowth, the cell wall must be modified to allow for cell expansion. Organ initiation is closely linked to, and regulated by, distributions of the plant hormone auxin. The cell wall polysaccharide, pectin, is modified at the sites of auxin accumulation and is required for organ outgrowth, at least in some plants. Previous work in maize showed that floral meristems have differing localization of pectin that may coincide with differences in organ fate. We characterized meristems in the female inflorescence which give rise to different structures and different number of primordia. Mutants with altered floral meristem determinacy were assessed to determine if changes in floral meristem activity correlated with changes in pectin localization. The shoot apical meristem was assessed, and we found that pectin localization is not uniform between vegetative meristems and floral meristems. We found that meristems of the inflorescence localize pectin differently and that pectin modification is necessary for some organ development. Mutants that lose determinacy localize pectin differently which suggests that pectin composition in the cell wall is intimately linked to proper meristem activity and floral organ primordia formation.
General note	Presented to the Faculty of the Biology Department
General note	Advisor: Beth Thompson
General note	Title from PDF t.p. (viewed May 13, 2024).
Dissertation note	M.S. East Carolina University 2022.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

Characterizing the role of pectin in cell wall composition and organ initiation in maize / by Daniel Jarrell Maynard.

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