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001 on1397376825;

003 OCoLC;

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007 cr unu||||||||;

008 230915s2023 ncua obm 000 0 eng d;

035 a| (Sirsi) o1397376825;

035 a| (OCoLC)1397376825;

049 a| EREE;

090 a| RC116.S8;

100 1 a| Norman, Mina,e| author.?| UNAUTHORIZED;

245 14 a| The effects of the unnatural amino acid TTO-53 on the biofilm of staphylococcus aureus /c| by Mina Norman.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2023.;

300 a| 1 online resource (38 pages) :b| color illustrations;

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 615.6 KB2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2023.;

502 a| Presented to the Faculty of the Department of Biology;

500 a| Advisor: Eric Anderson;

500 a| Title from PDF t.p. (viewed October 29, 2024).;

520 3 a| There are over 30 different species of staphylococci and many cause infection that can lead to hospitalization or death. The member most associated with chronic infection is Staphylococcus aureus. Staphylococcus aureus is a Gram-positive bacterium and usually harmless. It can be found on the skin of 15-40% of people in America. S. aureus spreads through contact with contaminated surfaces and skin to skin contact. Additionally, S. aureus has evolved a number of strategies to become more resistant to antibiotics. One strategy important to facilitating antibiotic resistance and resisting unfavorable environmental changes is biofilm. Biofilm is an extracellular matrix made up of extracellular DNA, proteins, and polysaccharides and acts as extra protection for the bacteria cells. Biofilm development happens in three phases, attachment, maturation, and dispersal phase. During attachment, cells aggregate due to poly-N-acetylglucosamine introducing a positive charge to the negatively charged bacteria. Cells will mature and aggregate through cell-to-cell communication with the use of autoinducer, using a regulatory system known as quorum sensing. Quorum sensing is regulated by a large number of genes involved in both assembly and disassembly. We have previously demonstrated that an unnatural amino acid, TTO-53 is able to disrupt biofilm in a several other organisms. The goal of this research was to determine if TTO- 53 demonstrates similar effects on the biofilm of Staphylococcus aureus and how this is occurring. Our data indicated that there are disruptive effects occurring in biofilm when TTO-53 is introduced to the environment. This disruption is not due to cell death. There is evidence that TTO-53 is impacting regulation of several biofilm genes. But it is still unknown how TTO-53 is causing disruption.;

504 a| Includes bibliographical references.;

650 0 a| Staphylococcus aureus infectionsx| Treatment.=| ^A819393;

650 0 a| Biofilms.=| ^A251023;

650 0 a| Pseudomonas aeruginosa.=| ^A717;

650 0 a| Amino acids.=| ^A6734;

653 a| antimicrobial peptide;

653 a| TTO-53;

653 a| quorum sensing;

653 a| Accessory Gene Regulatory System;

653 a| unnatural amino acids;

700 1 a| Anderson, Eric,e| degree supervisor.=| ^A696706;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/13146;

949 o| wjh;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 6234538;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 6234538-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 9/15/2023x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 6234538-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 9/15/2023x| ETDz| HERESOURCE;

The effects of the unnatural amino acid TTO-53 on the biofilm of staphylococcus aureus / by Mina Norman.

Author/creator	Norman, Mina author.
Other author	Anderson, Eric, degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2023.
Description	1 online resource (38 pages) : color illustrations
Supplemental Content	Access via ScholarShip
Subjects	Staphylococcus aureus infections--Treatment. Biofilms. Pseudomonas aeruginosa. Amino acids.

Summary	There are over 30 different species of staphylococci and many cause infection that can lead to hospitalization or death. The member most associated with chronic infection is Staphylococcus aureus. Staphylococcus aureus is a Gram-positive bacterium and usually harmless. It can be found on the skin of 15-40% of people in America. S. aureus spreads through contact with contaminated surfaces and skin to skin contact. Additionally, S. aureus has evolved a number of strategies to become more resistant to antibiotics. One strategy important to facilitating antibiotic resistance and resisting unfavorable environmental changes is biofilm. Biofilm is an extracellular matrix made up of extracellular DNA, proteins, and polysaccharides and acts as extra protection for the bacteria cells. Biofilm development happens in three phases, attachment, maturation, and dispersal phase. During attachment, cells aggregate due to poly-N-acetylglucosamine introducing a positive charge to the negatively charged bacteria. Cells will mature and aggregate through cell-to-cell communication with the use of autoinducer, using a regulatory system known as quorum sensing. Quorum sensing is regulated by a large number of genes involved in both assembly and disassembly. We have previously demonstrated that an unnatural amino acid, TTO-53 is able to disrupt biofilm in a several other organisms. The goal of this research was to determine if TTO- 53 demonstrates similar effects on the biofilm of Staphylococcus aureus and how this is occurring. Our data indicated that there are disruptive effects occurring in biofilm when TTO-53 is introduced to the environment. This disruption is not due to cell death. There is evidence that TTO-53 is impacting regulation of several biofilm genes. But it is still unknown how TTO-53 is causing disruption.
General note	Advisor: Eric Anderson
General note	Title from PDF t.p. (viewed October 29, 2024).
Dissertation note	M.S. East Carolina University 2023.
Dissertation note	Presented to the Faculty of the Department of Biology
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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The effects of the unnatural amino acid TTO-53 on the biofilm of staphylococcus aureus / by Mina Norman.

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