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001 on1397376889;

003 OCoLC;

005 20260527041731.0;

006 m o d ;

007 cr unu||||||||;

008 230915s2023 ncua obm 000 0 eng d;

035 a| (OCoLC)1397376889;

090 a| SB191.M2;

049 a| EREE;

100 1 a| Rispress, Kimberly,e| author.;

245 10 a| Understanding the classical maize floral development mutant, Polytypic ear1 /c| by Kimberly Rispress.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2023.;

300 a| 1 online resource (58 pages) :b| illustrations (some color);

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| PDFc| 7.402 MB2| rda;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 b| M.S.c| East Carolina Universityd| 2023.;

500 a| Presented to the Faculty of the Biology Department;

500 a| Advisor: Beth Thompson;

500 a| Title from PDF t.p. (viewed November 20, 2024).;

520 3 a| Maize produces two inflorescences, the tassel and ear, which are critical for both plant reproduction and agriculture. The classical semi-dominant mutant, Polytypic ear1 (Pt1), affects multiple aspects of inflorescence development, including floral development. Pt1 heterozygote ears and tassels appear to have barren tips with extra floral organs towards the base with extra silks or branch-like protrusions. While the causative gene is unknown, RNA-seq provided direction for candidate gene analysis. Our top candidate gene, ethylene response sensor1 (ers1), encodes a putative ethylene receptor that shows allele-specific expression in Pt1. Ethylene receptors negatively regulate ethylene signaling, and in Arabidopsis, dominant mutations confer ethylene insensitivity. I hypothesized that overexpression of ethylene receptors may also confer a similar ethylene insensitive phenotype. To test this hypothesis, I evaluated ethylene sensitivity of Pt1 mutants. Pt1/+ siblings had no significant difference in growth in the presence and absence of the ethylene precursor ACC, but these results are not conclusive due to lack of robust wildtype results. While the ers1 RNA in situ hybridization failed to detect ers1 transcripts, the in situs of genes expressed in the boundary between upper and lower florets were similar in normal and Pt1/+ plants. The pectin localization patterns of Pt1/+ plants differed from normal siblings suggesting that floral meristem determinacy could be linked to changes in cell wall dynamics.;

504 a| Includes bibliographical references.;

650 0 a| Cornx| Development.;

650 0 a| Cornx| Genetics.;

650 0 a| Inflorescencesx| Development.;

650 0 a| Plant mutation.;

653 a| maize;

653 a| floral development mutant;

700 1 a| Thompson, Beth E.,e| degree supervisor.;

710 2 a| East Carolina University.b| Department of Biology.;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/13139;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 6234549;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 6234549-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 9/15/2023x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 6234549-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 9/15/2023x| ETDz| HERESOURCE;

Understanding the classical maize floral development mutant, Polytypic ear1 / by Kimberly Rispress.

Author/creator	Rispress, Kimberly author.
Other author	Thompson, Beth E., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2023.
Description	1 online resource (58 pages) : illustrations (some color)
Supplemental Content	Access via ScholarShip
Subjects	Corn--Development. Corn--Genetics. Inflorescences--Development. Plant mutation.

Summary	Maize produces two inflorescences, the tassel and ear, which are critical for both plant reproduction and agriculture. The classical semi-dominant mutant, Polytypic ear1 (Pt1), affects multiple aspects of inflorescence development, including floral development. Pt1 heterozygote ears and tassels appear to have barren tips with extra floral organs towards the base with extra silks or branch-like protrusions. While the causative gene is unknown, RNA-seq provided direction for candidate gene analysis. Our top candidate gene, ethylene response sensor1 (ers1), encodes a putative ethylene receptor that shows allele-specific expression in Pt1. Ethylene receptors negatively regulate ethylene signaling, and in Arabidopsis, dominant mutations confer ethylene insensitivity. I hypothesized that overexpression of ethylene receptors may also confer a similar ethylene insensitive phenotype. To test this hypothesis, I evaluated ethylene sensitivity of Pt1 mutants. Pt1/+ siblings had no significant difference in growth in the presence and absence of the ethylene precursor ACC, but these results are not conclusive due to lack of robust wildtype results. While the ers1 RNA in situ hybridization failed to detect ers1 transcripts, the in situs of genes expressed in the boundary between upper and lower florets were similar in normal and Pt1/+ plants. The pectin localization patterns of Pt1/+ plants differed from normal siblings suggesting that floral meristem determinacy could be linked to changes in cell wall dynamics.
General note	Presented to the Faculty of the Biology Department
General note	Advisor: Beth Thompson
General note	Title from PDF t.p. (viewed November 20, 2024).
Dissertation note	M.S. East Carolina University 2023.
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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