Characterization of the [beta]-2 microglobulin gene in channel catfish (Ictalurus punctatus) / by Ralph G. Benedetto, Jr.
| Author/creator | Benedetto, Ralph G. author. |
| Other author | McConnell, Thomas J., degree supervisor. |
| Other author | East Carolina University. Department of Biology. |
| Format | Theses and dissertations |
| Production | 1995. |
| Description | 41 leaves : illustrations ; 28 cm |
| Supplemental Content | Access via ScholarShip |
| Subjects |
| Summary | The Major Histocompatibility Complex (MHC) is a group of highly polymorphic genes that codes for cell surface glycoproteins that present foreign antigens to T cells. There are two classes of MHC molecules, each consisting of two noncovalently associated glycoprotein chains. The two peptides which make up the MHC Class II molecule are similar in size and structure, and both are encoded by genes within the MHC in higher vertebrates. The two peptides which make up the Class I molecule differ in size and structure, with the larger a chain encoded by a gene within the higher vertebrate MHC and the smaller /3-2 microglobulin encoded by a gene not found within the MHC in higher vertebrates. Structural similarities between the glycoprotein chains of both classes of MHC molecules indicate that they share a common evolutionary origin, but the process by which these molecules arose is still unclear. The j8-2 microglobulin gene in Ictalurus punctatus was isolated from a cDNA library using a PCR derived probe amplified using primers originally designed around conserved regions in the Class II a-chain gene and sequenced. Both the nucleotide sequence and the amino acid sequence derived from that nucleotide sequence were compared to sequences from other teleosts and higher vertebrates. Phylogenetic analysis failed to support any of the current theories regarding the development of the MHC but did give some indications that the teleost MHC may have developed along different lines than the MHC in higher vertebrates. |
| General note | Presented to the Faculty of the Department of Biology |
| General note | Advisor: Thomas J. McConnell |
| General note | Submitted to the faculty of the Department of Biology. |
| Dissertation note | M.S. East Carolina University 1995 |
| Bibliography note | Includes bibliographical references (leaves 38-41). |
| Genre/form | Academic theses. |
| Genre/form | Academic theses. |
| Genre/form | Thèses et écrits académiques. |
Availability
| Library | Location | Call Number | Status | Item Actions |
|---|---|---|---|---|
| Joyner | University Archives | ASK AT SPECIAL COLLECTIONS DESK | ✔ Available | Request Material |
| Electronic Resources | Access Content Online | ✔ Available |