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001 ocm39055702;

003 OCoLC;

005 20250422153007.0;

008 980504s1997 xx a bm 000 0 eng d;

035 a| (Sirsi) o39055702;

035 a| (OCoLC)39055702;

049 a| EREE;

050 4 a| RC585.5b| .L33 1997;

100 1 a| Lam, Johnsimon K.,e| author.?| UNAUTHORIZED;

245 10 a| Immunomodulation of TH1 and TH2 cytokine gene expression by IL-12 in allergic mice /c| by Johnsimon K. Lam.;

264 0 c| 1997.;

300 a| 58 leaves :b| illustrations ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 1997;

500 a| Submitted to the faculty of the Department of Biology.;

500 a| Advisor: W. James Metzger;

520 3 a| The effects of timing and route of administration of IL-12 on murine cytokine expression in allergic airway inflammation were investigated. Administration of IL-12 was timed to interfere with either allergic sensitization (early dosage) or the hypersensitivity inflammatory response in the lung (late dosage), or both (early and late dosage). Comparisons of IL-12 and PBS-treated animals within each group revealed significant effects of IL-12. Early dosage down-regulated IL-5, IL-10, but did not change IL-4 or IFN-y gene expression. Late dosage down-regulated IL-5, up-regulated IL-10 and IFN- y but did not change IL-4 gene expression. The combination of early and late dosage down-regulated IL-4, IL 5, and IL-10 expression, but increased IFN-y gene expression. Administration of IL-12 via mucosal and systemic injections were performed. Comparisons of mucosal and systemic groups revealed that mucosal IL-12 up-regulated IFN-y and IL-10, but did not alter lL-4 and lL-5 gene expression in BAL cells. In contrast, systemic IL-12 inhibited IL-4 and IL-5, but did not alter IL-10 and IFN-y gene expression. These results suggest IL-12 has potent immunomodulatory effects on allergic lung inflammation that depend on the timing and route of IL-12 administration.;

504 a| Includes bibliographical references (leaves 49-55).;

650 0 a| Allergyx| Research.=| ^A818;

650 0 a| Allergy in animals.=| ^A398933;

650 0 a| Immunological adjuvants.=| ^A286441;

650 0 a| Lungsx| Diseases.=| ^A1248;

650 0 a| Mice as laboratory animals.=| ^A848;

650 2 a| Adjuvants, Immunologic.0| (DNLM)D000276=| ^A926942;

650 2 a| Lung Diseases.0| (DNLM)D008171=| ^A917918;

655 2 a| Academic Dissertation.0| (DNLM)D019478?| UNAUTHORIZED;

650 7 a| Allergy in animals.2| fast0| (OCoLC)fst00805573;

650 7 a| Allergyx| Research.2| fast0| (OCoLC)fst00805567;

650 7 a| Immunological adjuvants.2| fast0| (OCoLC)fst00967992;

650 7 a| Lungsx| Diseases.2| fast0| (OCoLC)fst01003777;

650 7 a| Mice as laboratory animals.2| fast0| (OCoLC)fst01019388;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Metzger, W. James,e| degree supervisor.1| https://id.oclc.org/worldcat/entity/E39PCjv7Qwt6fxKh3BR8pQW4bd=| ^A955023;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/12554;

949 a| Click on web addressw| asish| joyner101;

949 a| Click on web addressw| asish| hsl111;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 630083;

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Immunomodulation of TH1 and TH2 cytokine gene expression by IL-12 in allergic mice / by Johnsimon K. Lam.

Author/creator	Lam, Johnsimon K. author.
Other author	Metzger, W. James, degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	1997.
Description	58 leaves : illustrations ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Allergy--Research. Allergy in animals. Immunological adjuvants. Lungs--Diseases. Mice as laboratory animals. Adjuvants, Immunologic. Lung Diseases.

Summary	The effects of timing and route of administration of IL-12 on murine cytokine expression in allergic airway inflammation were investigated. Administration of IL-12 was timed to interfere with either allergic sensitization (early dosage) or the hypersensitivity inflammatory response in the lung (late dosage), or both (early and late dosage). Comparisons of IL-12 and PBS-treated animals within each group revealed significant effects of IL-12. Early dosage down-regulated IL-5, IL-10, but did not change IL-4 or IFN-y gene expression. Late dosage down-regulated IL-5, up-regulated IL-10 and IFN- y but did not change IL-4 gene expression. The combination of early and late dosage down-regulated IL-4, IL 5, and IL-10 expression, but increased IFN-y gene expression. Administration of IL-12 via mucosal and systemic injections were performed. Comparisons of mucosal and systemic groups revealed that mucosal IL-12 up-regulated IFN-y and IL-10, but did not alter lL-4 and lL-5 gene expression in BAL cells. In contrast, systemic IL-12 inhibited IL-4 and IL-5, but did not alter IL-10 and IFN-y gene expression. These results suggest IL-12 has potent immunomodulatory effects on allergic lung inflammation that depend on the timing and route of IL-12 administration.
General note	Submitted to the faculty of the Department of Biology.
General note	Advisor: W. James Metzger
Dissertation note	M.S. East Carolina University 1997
Bibliography note	Includes bibliographical references (leaves 49-55).
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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