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035 a| (Sirsi) o39487095;

035 a| (OCoLC)39487095;

049 a| EREE;

050 4 a| QR186b| .A76 1997;

100 1 a| Armacost, J. L.,e| author.?| UNAUTHORIZED;

245 10 a| Characteristics of the immune response to type III pneumococcal polysaccharide induced in BALB/c mice through the use of immunostimulating complexes /c| by J.L. Armacost.;

264 0 c| 1997.;

300 a| 99 leaves :b| illustrations ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 1997;

500 a| Submitted to the faculty of the Department of Biology.;

500 a| Advisor: A. Mason Smith;

520 3 a| This research was designed to study the immune response in BALB/c mice to a type III pneumoccocal capsular polysaccharide-bovine serum albumin (BSA) conjugate incorporated in immunostimulating complexes (ISCOMs). The study included the distribution of the immunogen via different routes while monitoring both the systemic and mucosal responses. This work was based on the original hypothesis that a polysaccharide-carrier protein conjugate incorporated into ISCOMs would (1) induce an anamnestic response consisting primarily of IgG antibodies when delivered subcutaneously (s.q.) and intraperitoneally (i.p ), and (2) generate an IgA response when given orally to BALB/c mice. The humoral immune responses were monitored in serum and intestinal gavages by ELISA and PHA. The quantification of splenocytes producing antibody specific for type III polysaccharide was done by ELISPOT. Peyer's patch cells were characterized after staining thin sections with hematoxylin and eosin. In preliminary studies, type III pneumococcal polysaccharide was conjugated with BSA using two different chemical reagents; 4-(maleimidomethyl) cyclohexane 1- Carboxyl hydrazide ( M2C2H) and cyanuric chloride (C3CI3N3). The presence of the ISCOMs structure was verified by electron microscopy, and its incorporated conjugate by carbohydrate assay and protein assay. ISCOMs injected i.p. and s.q. induced both an IgM and IgG systemic response. The IgG response was shown to be continuous over several weeks. Data obtained by ELISPOT indicated that the spleen was a major source of antibodies produced. Therefore, presented evidence supports the induction of an anamnestic response when the type III polysaccharide was given as part of the ISCOM vehicle. Oral feeding of the polysaccharide in the ISCOM vehicle did not produce secretory IgA in the intestine as measured in this study. However, oral feeding did produce a systemic IgG response and significant changes in Peyer's patch cell populations. These findings are discussed along with the importance of ISCOMs as a means of inducing protective immunity against some common bacterial polysaccharides.;

504 a| Includes bibliographical references (leaves 78-86).;

650 0 a| Immune response.=| ^A1311;

650 0 a| Serum albumin.=| ^A14954;

650 0 a| Mice as laboratory animals.=| ^A848;

650 2 a| Serum Albumin0| (DNLM)D012709=| ^A950563;

655 2 a| Academic Dissertation.0| (DNLM)D019478?| UNAUTHORIZED;

650 7 a| Immune response.2| fast0| (OCoLC)fst00967867;

650 7 a| Mice as laboratory animals.2| fast0| (OCoLC)fst01019388;

650 7 a| Serum albumin.2| fast0| (OCoLC)fst01113386;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Smith, A. Mason,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 41 z| Access via ScholarShipu| http://hdl.handle.net/10342/11466;

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Characteristics of the immune response to type III pneumococcal polysaccharide induced in BALB/c mice through the use of immunostimulating complexes / by J.L. Armacost.

Author/creator	Armacost, J. L. author.
Other author	Smith, A. Mason, degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	1997.
Description	99 leaves : illustrations ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Immune response. Serum albumin. Mice as laboratory animals. Serum Albumin

Summary	This research was designed to study the immune response in BALB/c mice to a type III pneumoccocal capsular polysaccharide-bovine serum albumin (BSA) conjugate incorporated in immunostimulating complexes (ISCOMs). The study included the distribution of the immunogen via different routes while monitoring both the systemic and mucosal responses. This work was based on the original hypothesis that a polysaccharide-carrier protein conjugate incorporated into ISCOMs would (1) induce an anamnestic response consisting primarily of IgG antibodies when delivered subcutaneously (s.q.) and intraperitoneally (i.p ), and (2) generate an IgA response when given orally to BALB/c mice. The humoral immune responses were monitored in serum and intestinal gavages by ELISA and PHA. The quantification of splenocytes producing antibody specific for type III polysaccharide was done by ELISPOT. Peyer's patch cells were characterized after staining thin sections with hematoxylin and eosin. In preliminary studies, type III pneumococcal polysaccharide was conjugated with BSA using two different chemical reagents; 4-(maleimidomethyl) cyclohexane 1- Carboxyl hydrazide ( M2C2H) and cyanuric chloride (C3CI3N3). The presence of the ISCOMs structure was verified by electron microscopy, and its incorporated conjugate by carbohydrate assay and protein assay. ISCOMs injected i.p. and s.q. induced both an IgM and IgG systemic response. The IgG response was shown to be continuous over several weeks. Data obtained by ELISPOT indicated that the spleen was a major source of antibodies produced. Therefore, presented evidence supports the induction of an anamnestic response when the type III polysaccharide was given as part of the ISCOM vehicle. Oral feeding of the polysaccharide in the ISCOM vehicle did not produce secretory IgA in the intestine as measured in this study. However, oral feeding did produce a systemic IgG response and significant changes in Peyer's patch cell populations. These findings are discussed along with the importance of ISCOMs as a means of inducing protective immunity against some common bacterial polysaccharides.
General note	Submitted to the faculty of the Department of Biology.
General note	Advisor: A. Mason Smith
Dissertation note	M.S. East Carolina University 1997
Bibliography note	Includes bibliographical references (leaves 78-86).
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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Characteristics of the immune response to type III pneumococcal polysaccharide induced in BALB/c mice through the use of immunostimulating complexes / by J.L. Armacost.

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