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001 ocm40998851;

003 OCoLC;

005 20240122153642.0;

008 990319s1998 xx a bm 000 0 eng d;

035 a| (Sirsi) o40998851;

035 a| (OCoLC)40998851;

049 a| EREE;

050 4 a| QP93b| .C52 1998;

100 1 a| Ciaramella, Jason,e| author.?| UNAUTHORIZED;

245 10 a| Isolation, characterization and functional activity of human placental stromal cells in hematopoiesis /c| by Jason Ciaramella.;

264 0 c| 1998.;

300 a| 59 leaves :b| illustrations ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 1998;

500 a| Submitted to the faculty of the Department of Biology.;

500 a| Advisor: George Sigounas;

520 3 a| The purpose of this study was to determine the ability of human placental stromal cells (PLSC) to support the survival and growth of CD34+ cells. To address this goal, PLSCs were isolated from human full term placenta. CD34+ cells were isolated from human umbilical cord blood (UCB) mononuclear cells (MNC) and co-cultured with primary PLSCs and several PLSC lines. Culture of human UCB CD34+ stem cells with PLSC resulted in the self renewal, proliferation, and differentiation of colony forming unit-granulocyte erythroid megakaryocyte monocyte (CFU-GEMM) and colony forming unit-granulocyte monocyte (CFU-GM) progenitor cells. Location of the growth of CD34+ cells co-cultured with PLSCs was analyzed. In addition, humoral factors produced by the PLSCs were examined for ability to support the growth and survival of CD34+ progenitor cells. Finally, the ability of PLSCs to support the survival and growth of CD34-I- progenitor cells in serum free conditions was tested. Collectively, these results support the hypothesis that the human placenta contains distinct stromal cells with varying abilities to support the self-renewal, proliferation and differentiation of CD34+ progenitor cells.;

504 a| Includes bibliographical references (leaves 56-59).;

650 0 a| Bloodx| Analysis.=| ^A230982;

650 0 a| Hematopoiesis.=| ^A56193;

650 7 a| Bloodx| Analysis.2| fast0| (OCoLC)fst00834552;

650 7 a| Hematopoiesis.2| fast0| (OCoLC)fst00954922;

655 7 a| dissertations.2| aat0| (CStmoGRI)aatgf300028029;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Sigounas, George,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/11911;

949 a| Click on web addressw| asish| joyner101;

949 a| Click on web addressw| asish| hsl111;

994 a| C0b| ERE;

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998 a| 646647;

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Isolation, characterization and functional activity of human placental stromal cells in hematopoiesis / by Jason Ciaramella.

Author/creator	Ciaramella, Jason author.
Other author	Sigounas, George, degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	1998.
Description	59 leaves : illustrations ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Blood--Analysis. Hematopoiesis.

Summary	The purpose of this study was to determine the ability of human placental stromal cells (PLSC) to support the survival and growth of CD34+ cells. To address this goal, PLSCs were isolated from human full term placenta. CD34+ cells were isolated from human umbilical cord blood (UCB) mononuclear cells (MNC) and co-cultured with primary PLSCs and several PLSC lines. Culture of human UCB CD34+ stem cells with PLSC resulted in the self renewal, proliferation, and differentiation of colony forming unit-granulocyte erythroid megakaryocyte monocyte (CFU-GEMM) and colony forming unit-granulocyte monocyte (CFU-GM) progenitor cells. Location of the growth of CD34+ cells co-cultured with PLSCs was analyzed. In addition, humoral factors produced by the PLSCs were examined for ability to support the growth and survival of CD34+ progenitor cells. Finally, the ability of PLSCs to support the survival and growth of CD34-I- progenitor cells in serum free conditions was tested. Collectively, these results support the hypothesis that the human placenta contains distinct stromal cells with varying abilities to support the self-renewal, proliferation and differentiation of CD34+ progenitor cells.
General note	Submitted to the faculty of the Department of Biology.
General note	Advisor: George Sigounas
Dissertation note	M.S. East Carolina University 1998
Bibliography note	Includes bibliographical references (leaves 56-59).
Genre/form	dissertations.
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

Isolation, characterization and functional activity of human placental stromal cells in hematopoiesis / by Jason Ciaramella.

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