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035 a| (Sirsi) o1506074728;

035 a| (OCoLC)1506074728;

049 a| EREE;

096 a| QU 476;

100 1 a| Berkuta, Jonathan,e| author.;

245 10 a| Characterizing the epigenetic and transcriptional regulation of the conjoined CHKB-CPT1B locus /c| by Jonathan Berkuta.;

264 1 a| [Greenville, N.C.] :b| [East Carolina University],c| 2024.;

300 a| 1 online resource (194 pages) :b| illustrations (chiefly color).;

336 a| textb| txt2| rdacontent;

337 a| computerb| c2| rdamedia;

338 a| online resourceb| cr2| rdacarrier;

347 a| text fileb| application/pdfc| 5.93 MB2| rda;

490 1 a| ECU Brody School of Medicine dissertation;

538 a| System requirements: Adobe Reader.;

538 a| Mode of access: World Wide Web.;

502 c| East Carolina Universityd| 2024.;

502 a| Presented to the Faculty of the Department of Biochemistry and Molecular Biology;

500 a| Advisor: Brian Shewchuk;

500 a| Title from PDF t.p. (viewed March 5, 2026).;

520 3 a| The emergence of brown adipose tissue was an important event for vertebrate development; however, much is still unknown about this emergence. Previously, our lab showed that, in placental mammals, the Chkb and Cpt1b genes are conjoined and regulated by a singular upstream promoter region, whereas an intergenic CpG island prevents the conjunction of these genes in marsupials. Chkb encodes for choline kinase B (CHKB), which catalyzes the first step in phosphatidylcholine synthesis, while Cpt1b encodes for carnitine palmitoyltransferase 1B (CPT1B), which is the rate-limiting enzyme in fatty acid oxidation. We believe the loss of the marsupial intergenic CGI led to the conjunction of the Chkb-Cpt1b gene locus, and that CHKB is necessary for the brown adipocyte phenotype. In addition, we also believe lactylation, a novel form of epigenetic modification, is present across the Chkb-Cpt1b locus. In this dissertation, we show the intergenic CGI possesses enhancer and weak promoter activity and that CHKA, and not CHKB, is responsible for the majority of phosphatidylcholine synthesis in C2C12 and DE2.3 cells. We also show the presence of lactylation in these cells. We hope this data can be used to further our understanding of the emergence of brown adipocytes and provide future therapies to various diseases such as obesity.;

504 a| Includes bibliographical references.;

650 2 a| Epigenesis, Genetic.;

653 a| Brown adipocytes;

653 a| Epigenetic regulation;

653 a| CHKB;

653 a| CPT1B;

653 a| Conjoined genes;

653 a| Lactylation;

700 1 a| Shewchuk, Brian M.,e| degree supervisor.;

710 2 a| East Carolina University.b| Department of Biochemistry and Molecular Biology.;

830 0 a| ECU Brody School of Medicine dissertation.;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/13855;

949 o| wjh;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 6560823;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 6560823-1001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 3/10/2025x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 6560823-2001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 3/10/2025x| ETDz| HERESOURCE;

Characterizing the epigenetic and transcriptional regulation of the conjoined CHKB-CPT1B locus / by Jonathan Berkuta.

Author/creator	Berkuta, Jonathan author.
Other author	Shewchuk, Brian M., degree supervisor.
Other author	East Carolina University. Department of Biochemistry and Molecular Biology.
Format	Theses and dissertations
Publication	[Greenville, N.C.] : [East Carolina University], 2024.
Description	1 online resource (194 pages) : illustrations (chiefly color).
Supplemental Content	Access via ScholarShip
Subjects	Epigenesis, Genetic.

Series	ECU Brody School of Medicine dissertation ECU Brody School of Medicine dissertation.
Summary	The emergence of brown adipose tissue was an important event for vertebrate development; however, much is still unknown about this emergence. Previously, our lab showed that, in placental mammals, the Chkb and Cpt1b genes are conjoined and regulated by a singular upstream promoter region, whereas an intergenic CpG island prevents the conjunction of these genes in marsupials. Chkb encodes for choline kinase B (CHKB), which catalyzes the first step in phosphatidylcholine synthesis, while Cpt1b encodes for carnitine palmitoyltransferase 1B (CPT1B), which is the rate-limiting enzyme in fatty acid oxidation. We believe the loss of the marsupial intergenic CGI led to the conjunction of the Chkb-Cpt1b gene locus, and that CHKB is necessary for the brown adipocyte phenotype. In addition, we also believe lactylation, a novel form of epigenetic modification, is present across the Chkb-Cpt1b locus. In this dissertation, we show the intergenic CGI possesses enhancer and weak promoter activity and that CHKA, and not CHKB, is responsible for the majority of phosphatidylcholine synthesis in C2C12 and DE2.3 cells. We also show the presence of lactylation in these cells. We hope this data can be used to further our understanding of the emergence of brown adipocytes and provide future therapies to various diseases such as obesity.
General note	Advisor: Brian Shewchuk
General note	Title from PDF t.p. (viewed March 5, 2026).
Dissertation note	East Carolina University 2024.
Dissertation note	Presented to the Faculty of the Department of Biochemistry and Molecular Biology
Bibliography note	Includes bibliographical references.
Technical details	System requirements: Adobe Reader.
Technical details	Mode of access: World Wide Web.

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Characterizing the epigenetic and transcriptional regulation of the conjoined CHKB-CPT1B locus / by Jonathan Berkuta.

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