ELISA advances in research and applications / Jose Ma. M. Angeles, editor.
| Other author | Angeles, Jose Ma. M. |
| Format | Electronic |
| Publication Info | New York : Nova Medicine & Health, [2022] |
| Description | viii, 242 pages ; 23 cm. |
| Supplemental Content | Full text available from Ebook Central - Academic Complete |
| Subjects |
| Series | Medical procedures, testing and technology |
| Contents | Updates in the diagnostic use of ELISA for schistosomiasis / Kevin Austin L. Ona and Jose Ma. M. Angeles -- Use of an ELISA to assess the immunogenicity and protective potential of a Babesia bigemina RAP-1, GP45, and 12D3 recombinant protein cocktail in cattle / Rebeca M. Santamaria-Espinosa, José J. Lira-Amaya, Patricia VargasUrióstegui, Roberto O. Castaneda-Ríos, Grecia Martínez-García, J. Antonio Álvarez-Martínez, Carmen Rojas-Martínez and Julio V. Figueroa-Millán -- ELISA test using Babesia Sp. Recombinant antigens for epidemiological diagnosis of Bovine babesiosis in Mexico / Julio V. Figueroa-Millán, Roberto O. Castañeda-Ríos, José J. Lira-Amaya, Patricia Vargas-Urióstegui, J. Antonio Álvarez-Martínez and Carmen Rojas-Martínez -- ELISA : a sero-surveillance tool in malaria elimination / Pilarita T. Rivera -- Applications of enzyme-linked immunosorbent assays in functional food development and regulation / Deborah S. Nicdao, Princess Alyssa S. Abid, Mia Beatriz C. Amoranto, Joseph Q. Bechayda, Sharah Kristine G. Bonguit, Calline Danica D. Gomez and Marilen Parungao Balolong -- A refined rapid antibody-based immunoassay improved sensitivity of paralytic shellfish poisoning toxins detection / Chun-Kwan Wong, Pui-Yi Cheung and Rickjason Chi-Wai Chan -- Aptamers : the chemical antibodies, their emergence, and application to enzyme-linked immunosorbent assay / Altair B. Agmata, Chembie A. Almazar, Marjo V. Mendoza and Windell L. Rivera. |
| Abstract | "In September 1971, Swiss scientists Eva Engvall and Peter Perlmann published a communication in the journal Immunochemistry on enzyme-linked immunosorbent assay (ELISA) as a quantitative assay of immunoglobulin G. They have modified radioimmunoassay (RIA) by replacing the radioactive iodine with enzymes and used in determining the levels of IgG in rabbit serum. Fifty years after, ELISA is still the most commonly used technique in measuring peptides, proteins, antibodies, antigens, and hormones in various samples. This plate-based assay is a very versatile laboratory technique and offers many advantages to laboratory scientists and researchers such as generating quick results, simple protocols, high sensitivity, being easily automated and being convenient to perform. Its application in the diagnosis of different infectious diseases has shown the unquestionable importance of this technique even in the modern world. In this book, the usefulness of ELISA in the control of different parasitic diseases including schistosomiasis, babesiosis, and malaria will be discussed. There is also a chapter featuring an extensive discussion on the use of ELISA in functional food development and regulation. And finally, the last two chapters will present advanced techniques inspired by ELISA including a rapid immunoassay for detecting paralytic shellfish poisoning toxins and the enzyme-linked aptamer assay. May this book provide significant information regarding the current advances and applications of ELISA and possibly inspire its use in another 50 years"-- Provided by publisher. |
| Bibliography note | Includes bibliographical references and index. |
| Access restriction | Available only to authorized users. |
| Technical details | Mode of access: World Wide Web |
| Genre/form | Electronic books. |
| LCCN | 2022002110 2021354878 |
| ISBN | 9781685075002 (hardcover) |
| ISBN | (adobe pdf) |