Tools

LEADER 03926ctm 2200565 i 4500

001 ocm43621545;

003 OCoLC;

005 20231106091053.0;

008 000313s1999 xx a bm 000 0 eng d;

035 a| (Sirsi) o43621545;

035 a| (OCoLC)43621545;

049 a| EREE;

050 4 a| QH603.M5b| B66 1999;

100 1 a| Bonner, Diane S.,e| author.?| UNAUTHORIZED;

245 10 a| Mapping of the human mitochondrial translation intiation factor 2 gene (MTIF2) to human chromosome 2 /c| by Diane S. Bonner.;

264 0 c| 1999.;

300 a| vi, 78 leaves :b| illustrations (some color) ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 1999;

500 a| Submitted to the faculty of the Department of Biology.;

500 a| Advisor: Mary A. Farwell;

520 3 a| Human Mitochondrial Translation Initiation Factor 2 (MTIF2) is a nuclear gene. Its product, IF2mt, is needed for the process of translation in mitochondria. IF2 is made on cytoplasmic ribosomes in the cytosol, and then imported into the mitochondrial matrix. It catalyzes the binding of IMet-tRNA to the ribosomal small subunit. Complete cDNAs encoding human IF2mt have been isolated from cDNA libraries, cloned and sequenced. (Ma and Spremulli, 1995). Several genomic clones containing MTIF2 have been isolated from a Lambda Fix" library and the DNA sequenced. The chromosomal location of the gene was found using a somatic cell hybrid panel. Each DNA sample contained mouse chromosomes and one intact human chromosome. The polymerase chain reaction (PCR) technique, using two PCR primers designed from MTIF2 sequence data showed the location of the gene to be on chromosome 2. The PCR primers anneal to DNA sequences flanking the intron/exon junction near the 3'-end of the gene. Human parental DNA served as a positive control and mouse genomic DNA as a negative control. The fluorescence in situ hybridization (FISH) technique confirmed the location. Four human genomic clones containing overlapping fragments were used as probes on human metaphase chromosome spreads. A more precise location of the gene was achieved by G-banding of the metaphase spreads used in FISH. MTIF2 was mapped to the short arm of chromosome 2 in the region 2pl6 to 2pl4 (Bonner et al., 1998).;

504 a| Includes bibliographical references (leaves 67-78).;

650 0 a| Mitochondria.=| ^A434;

650 0 a| Mitochondrial DNA.=| ^A348042;

650 0 a| DNA.=| ^A8939;

650 2 a| Mitochondria0| (DNLM)D008928=| ^A917755;

650 2 a| DNA, Mitochondrial0| (DNLM)D004272=| ^A920998;

650 2 a| DNA0| (DNLM)D004247=| ^A920250;

655 2 a| Academic Dissertation.0| (DNLM)D019478?| UNAUTHORIZED;

650 7 a| DNA.2| fast0| (OCoLC)fst00886555;

650 7 a| Mitochondria.2| fast0| (OCoLC)fst01024016;

650 7 a| Mitochondrial DNA.2| fast0| (OCoLC)fst01024018;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Farwell, Mary A.,e| degree supervisor.?| UNAUTHORIZED;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/11871;

949 a| Click on web addressw| asish| joyner101;

949 a| Click on web addressw| asish| hsl111;

994 a| C0b| ERE;

596 a| 1 4;

998 a| 722413;

999 a| ASK AT SPECIAL COLLECTIONS DESKw| ASISc| 1i| 722413-1001l| JSCJTHDm| JOYNERr| Ys| Yt| JSCJTHDu| 3/2/2022x| ETDz| JSPECCOLL;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 722413-3001l| JNETm| JOYNERr| Ys| Yt| JNE3ETDu| 9/27/2023x| ETDz| JERESOURCE;

999 a| CLICK ON WEB ADDRESSw| ASISc| 1i| 722413-4001l| HSLELECm| HSLr| Ys| Yt| HEETDu| 9/27/2023x| ETDz| HERESOURCE;

Mapping of the human mitochondrial translation intiation factor 2 gene (MTIF2) to human chromosome 2 / by Diane S. Bonner.

Author/creator	Bonner, Diane S. author.
Other author	Farwell, Mary A., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	1999.
Description	vi, 78 leaves : illustrations (some color) ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	Mitochondria. Mitochondrial DNA. DNA. Mitochondria DNA, Mitochondrial DNA

Summary	Human Mitochondrial Translation Initiation Factor 2 (MTIF2) is a nuclear gene. Its product, IF2mt, is needed for the process of translation in mitochondria. IF2 is made on cytoplasmic ribosomes in the cytosol, and then imported into the mitochondrial matrix. It catalyzes the binding of IMet-tRNA to the ribosomal small subunit. Complete cDNAs encoding human IF2mt have been isolated from cDNA libraries, cloned and sequenced. (Ma and Spremulli, 1995). Several genomic clones containing MTIF2 have been isolated from a Lambda Fix" library and the DNA sequenced. The chromosomal location of the gene was found using a somatic cell hybrid panel. Each DNA sample contained mouse chromosomes and one intact human chromosome. The polymerase chain reaction (PCR) technique, using two PCR primers designed from MTIF2 sequence data showed the location of the gene to be on chromosome 2. The PCR primers anneal to DNA sequences flanking the intron/exon junction near the 3'-end of the gene. Human parental DNA served as a positive control and mouse genomic DNA as a negative control. The fluorescence in situ hybridization (FISH) technique confirmed the location. Four human genomic clones containing overlapping fragments were used as probes on human metaphase chromosome spreads. A more precise location of the gene was achieved by G-banding of the metaphase spreads used in FISH. MTIF2 was mapped to the short arm of chromosome 2 in the region 2pl6 to 2pl4 (Bonner et al., 1998).
General note	Submitted to the faculty of the Department of Biology.
General note	Advisor: Mary A. Farwell
Dissertation note	M.S. East Carolina University 1999
Bibliography note	Includes bibliographical references (leaves 67-78).
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

Availability

Library	Location	Call Number	Status	Item Actions
Joyner	University Archives	ASK AT SPECIAL COLLECTIONS DESK	✔ Available	Request Material
	Electronic Resources	Access Content Online	✔ Available

Mapping of the human mitochondrial translation intiation factor 2 gene (MTIF2) to human chromosome 2 / by Diane S. Bonner.

Click here for more information about this title

Availability