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003 OCoLC;

005 20231110015234.0;

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049 a| EREE;

050 4 a| QH452.8b| .B77 1999;

100 1 a| Burnett, Wendy A.,e| author.?| UNAUTHORIZED;

245 10 a| Use of chromosome microdissection in conjunction with fluorescent in situ hybridization and the polymerase chain reaction to investigate the mechanism responsible for polymorphic ribosomal gene locations in hyla chrysoscelis /c| by Wendy A. Burnett.;

264 0 c| 1999.;

300 a| iv, 69 leaves :b| illustrations (some color) ;c| 28 cm;

336 a| textb| txt2| rdacontent;

337 a| unmediatedb| n2| rdamedia;

338 a| volumeb| nc2| rdacarrier;

502 b| M.S.c| East Carolina Universityd| 1999;

500 a| Submitted to the faculty of the Department of Biology.;

500 a| Advisor: John E. Wiley;

520 3 a| Hyla chrysoscelis has been found to be polymorphic in terms of the chromosomal locations of the 18S and 28S ribosomal genes. There are three basic groupings of H. chrysoscelis in North America. Those that have nucleolar organizing regions (NORs) on chromosome 6, those with NORs on chromosome 8, and those with NORs on chromosomes 1 and 10. By employing the novel method of chromosome microdissection in conjunction with polymerase chain reaction amplification and fluorescent in situ hybridization the mechanism for polymorphic NORs in H. chrysoscelis was investigated. DNA fragments were PCR-amplified using 18S ribosomal gene sequences for primers and hybridized back to metaphase chromosomes by FISH. Only those fragments that were known to contain NORs hybridized back to metaphase chromosomes by FISH. All other regions microdissected did not hybridize by FISH. These results lead us to the conclusion that ribosomal gene sequences probably do not exist in other regions of the genome and supporting the hypothesis that the mechanism responsible for polymorphic NORs is that the ribosomal genes are or act like mobile genetic elements. This study also found that contrary to reports in the literature no modification to standard cytogenetic protocols was needed to make FISH probes from microdissected chromosome fragments of H. chrysoscelis.;

504 a| Includes bibliographical references (leaves 59-69).;

650 0 a| In situ hybridization.=| ^A267902;

650 0 a| Microdissection.=| ^A444455;

650 0 a| DNA.=| ^A8939;

650 0 a| Hylidae.=| ^A358053;

650 7 a| DNA.2| fast0| (OCoLC)fst00886555;

650 7 a| Hylidae.2| fast0| (OCoLC)fst00965432;

650 7 a| In situ hybridization.2| fast0| (OCoLC)fst00968339;

650 7 a| Microdissection.2| fast0| (OCoLC)fst01019726;

655 7 a| Academic theses.2| fast0| (OCoLC)fst01726453;

655 7 a| Academic theses.2| lcgft;

655 7 a| Thèses et écrits académiques.2| rvmgf0| (CaQQLa)RVMGF-000001173;

700 1 a| Wiley, John E.,e| degree supervisor.=| ^A788626;

710 2 a| East Carolina University.b| Department of Biology.=| ^A637467;

856 40 z| Access via ScholarShipu| http://hdl.handle.net/10342/11892;

949 a| Click on web addressw| asish| joyner101;

949 a| Click on web addressw| asish| hsl111;

994 a| C0b| ERE;

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Use of chromosome microdissection in conjunction with fluorescent in situ hybridization and the polymerase chain reaction to investigate the mechanism responsible for polymorphic ribosomal gene locations in hyla chrysoscelis / by Wendy A. Burnett.

Author/creator	Burnett, Wendy A. author.
Other author	Wiley, John E., degree supervisor.
Other author	East Carolina University. Department of Biology.
Format	Theses and dissertations
Production	1999.
Description	iv, 69 leaves : illustrations (some color) ; 28 cm
Supplemental Content	Access via ScholarShip
Subjects	In situ hybridization. Microdissection. DNA. Hylidae.

Summary	Hyla chrysoscelis has been found to be polymorphic in terms of the chromosomal locations of the 18S and 28S ribosomal genes. There are three basic groupings of H. chrysoscelis in North America. Those that have nucleolar organizing regions (NORs) on chromosome 6, those with NORs on chromosome 8, and those with NORs on chromosomes 1 and 10. By employing the novel method of chromosome microdissection in conjunction with polymerase chain reaction amplification and fluorescent in situ hybridization the mechanism for polymorphic NORs in H. chrysoscelis was investigated. DNA fragments were PCR-amplified using 18S ribosomal gene sequences for primers and hybridized back to metaphase chromosomes by FISH. Only those fragments that were known to contain NORs hybridized back to metaphase chromosomes by FISH. All other regions microdissected did not hybridize by FISH. These results lead us to the conclusion that ribosomal gene sequences probably do not exist in other regions of the genome and supporting the hypothesis that the mechanism responsible for polymorphic NORs is that the ribosomal genes are or act like mobile genetic elements. This study also found that contrary to reports in the literature no modification to standard cytogenetic protocols was needed to make FISH probes from microdissected chromosome fragments of H. chrysoscelis.
General note	Submitted to the faculty of the Department of Biology.
General note	Advisor: John E. Wiley
Dissertation note	M.S. East Carolina University 1999
Bibliography note	Includes bibliographical references (leaves 59-69).
Genre/form	Academic theses.
Genre/form	Academic theses.
Genre/form	Thèses et écrits académiques.

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Use of chromosome microdissection in conjunction with fluorescent in situ hybridization and the polymerase chain reaction to investigate the mechanism responsible for polymorphic ribosomal gene locations in hyla chrysoscelis / by Wendy A. Burnett.

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